Fan Z F, Chen H Y, Liang X M, Li H F
Department of Plant Pathology and National Laboratories for Agrobiotechnology, China Agricultural University, Beijing, People's Republic of China.
Arch Virol. 2003 Apr;148(4):773-82. doi: 10.1007/s00705-002-0964-6.
The complete nucleotide sequence of the prevalent strain of a potyvirus isolated from maize in Beijing, China was determined and compared with other closely related potyviruses. The viral genome comprises 9595 nucleotides, excluding the poly (A) tail, and encodes a putative polyprotein of 3063 amino acid residues. Sequence comparison of the coat proteins showed that this isolate was most closely related to most other potyviral isolates infecting maize across China with identities of about 99% and thus represented the prevalent strain. It was also closely related to most isolates of Sugarcane mosaic virus (SCMV) infecting maize in Europe with maximum identity of about 95% at the amino acid level. The polyprotein sequence of the Beijing isolate shares identities of 98% with those of two other Chinese maize isolates and shares identity of 69% with Maize dwarf mosaic virus-Bulgarian isolate, respectively. Phylogenetic analyses of the sequences indicated that the Beijing isolate can be tentatively referred to as a prevalent strain of SCMV.
测定了从中国北京玉米中分离出的一种马铃薯Y病毒流行株的完整核苷酸序列，并与其他密切相关的马铃薯Y病毒进行了比较。该病毒基因组由9595个核苷酸组成（不包括poly(A)尾），编码一个由3063个氨基酸残基组成的推定多聚蛋白。外壳蛋白的序列比较表明，该分离株与中国其他大多数感染玉米的马铃薯Y病毒分离株关系最为密切，同一性约为99%，因此代表了流行株。它与欧洲大多数感染玉米的甘蔗花叶病毒（SCMV）分离株也密切相关，氨基酸水平上的最大同一性约为95%。北京分离株的多聚蛋白序列与另外两个中国玉米分离株的多聚蛋白序列同一性分别为98%，与玉米矮花叶病毒保加利亚分离株的同一性为69%。序列的系统发育分析表明，北京分离株可暂定为SCMV的一个流行株。
