Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
Electrophoresis. 2010 Jun;31(11):1833-41. doi: 10.1002/elps.201000046.
Although glycoproteomic studies provide unique opportunities for cancer research, it has been necessary to develop specific methods for analysis of oncologically interesting glycoproteins. We describe a general, multimethodological approach for quantitative glycoproteomic analysis of fucosylated glycoproteins in human blood serum. A total of 136 putative fucosylated glycoproteins were identified with very high confidence in three clinically relevant sample pools (N=5 for each), with a mean CV of 3.1% observed for replicate analyses. Two samples were collected from subjects diagnosed with esophagus disease states, high-grade dysplasia plus esophageal adenocarcinoma, while the third sample was representative of a disease-free condition. Some glycoproteins, observed to be significantly upregulated in esophageal adenocarcinoma, i.e. more than twofold higher than in the disease-free condition, are briefly discussed. Further investigation will be necessary to validate these findings; however, the method itself is demonstrated to be an effective tool for quantitative glycoproteomics of clinical samples.
尽管糖蛋白质组学研究为癌症研究提供了独特的机会，但有必要开发专门的方法来分析具有肿瘤学意义的糖蛋白。我们描述了一种通用的、多方法学的方法，用于定量分析人血清中糖基化糖蛋白的岩藻糖基化。在三个具有临床相关性的样本池中（每个样本 5 个重复），非常有信心地鉴定出了 136 种假定的岩藻糖基化糖蛋白，观察到重复分析的平均 CV 为 3.1%。两个样本取自被诊断为食管疾病状态（高级别异型增生加食管腺癌）的受试者，而第三个样本代表无疾病状态。简要讨论了一些在食管腺癌中观察到明显上调的糖蛋白，即比无疾病状态高两倍以上。需要进一步的研究来验证这些发现；然而，该方法本身被证明是一种用于临床样本定量糖蛋白质组学的有效工具。
