牛组织蛋白酶S的完整氨基酸序列及牛组织蛋白酶L的部分序列。

The complete amino acid sequence of bovine cathepsin S and a partial sequence of bovine cathepsin L.

作者信息

Ritonja A, Colić A, Dolenc I, Ogrinc T, Podobnik M, Turk V

机构信息

Department of Biochemistry, Jozef Stefan Institute, Ljubljana, Yugoslavia.

出版信息

FEBS Lett. 1991 Jun 3;283(2):329-31. doi: 10.1016/0014-5793(91)80620-i.

DOI:10.1016/0014-5793(91)80620-i

PMID:2044774

Abstract

The complete amino acid sequence of bovine spleen cathepsin S has been determined. The single-chain protein contains 217 residues and has a Mr of 23,682. The primary structure was determined by sequencing of native protein and the peptides obtained by proteolytic cleavage with beta-trypsin, papaya proteinase IV and by chemical cleavage with cyanogen bromide. Comparison of the amino terminal sequences of the heavy and the light chain of bovine cathepsin L with that of bovine cathepsin S clearly indicates that the enzymes are structurally different.

摘要

牛脾组织组织蛋白酶S的完整氨基酸序列已被测定。该单链蛋白含有217个氨基酸残基，分子量为23,682。其一级结构通过对天然蛋白、经β-胰蛋白酶和木瓜蛋白酶IV酶解得到的肽段以及经溴化氰化学裂解得到的肽段进行测序来确定。通过比较牛组织蛋白酶L重链和轻链的氨基末端序列与牛组织蛋白酶S的氨基末端序列，清楚地表明这两种酶在结构上是不同的。