Okorokov L A, Sysuev V A, Kulaev I S
Eur J Biochem. 1978 Feb;83(2):507-11. doi: 10.1111/j.1432-1033.1978.tb12117.x.
The uptake of 1 micrometer calcium into 6-h-old germination tubes of the fungus Phytophthora infestans follows Michaelis-Menten kinetics with a Km of 33 +/- 4 micrometer and a V of 0.3 nmol.min-1.(5 x 10(4) cells)-1. Uptake is inhibited by ruthenium red and lanthanum (both at 1 micrometer) and by the proton conductors 2,4-dinitrophenol (1 mM) and carbonylcyanide m-chlorophenylhydrazone and carbonylcyanide p-trifluoromethoxyphenylhydrazone (1--10 micrometer) and also by sodium azide. These data suggest that calcium uptake is dependent on energy and on a carrier. Calcium uptake is stimulated by pyrophosphate but not by ATP, orthophosphate, or polyphosphate. This stimulation is prevented by proton conductors or by incubation at 0 degrees C.
致病疫霉6小时龄萌发管对1微摩尔钙的摄取遵循米氏动力学，米氏常数（Km）为33±4微摩尔，最大反应速度（V）为0.3纳摩尔·分钟-1·（5×104个细胞）-1。摄取受到钌红和镧（均为1微摩尔）、质子导体2,4-二硝基苯酚（1毫摩尔）、羰基氰化物间氯苯腙和羰基氰化物对三氟甲氧基苯腙（1 - 10微摩尔）以及叠氮化钠的抑制。这些数据表明钙的摄取依赖于能量和载体。焦磷酸盐可刺激钙的摄取，但三磷酸腺苷、正磷酸盐或多磷酸盐则无此作用。这种刺激可被质子导体或在0℃孵育所阻止。
