人卵巢癌紫杉醇耐药细胞系的蛋白质组学分析
Proteomic analysis of human ovarian cancer paclitaxel-resistant cell lines.
作者信息
Cao Lanqin, Li Xin, Zhang Yi, Peng Fang, Yi Hong, Xu Yan, Li Xinguo, Wang Qian
机构信息
Department of Gynecology and Obstetrics, Xiangya Hospital, Central South University, Changsha 410008, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 Apr;35(4):286-94. doi: 10.3969/j.issn.1672-7347.2010.04.002.
OBJECTIVE
To study the proteins related to paclitaxel-resistant of ovarian cancer cell line.
METHODS
The total proteins of paclitaxel-resistant and paclitaxel-sensitive human ovarian cancer cell lines were separated by 2-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were analyzed using image analysis software. The differential proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins.
RESULTS
Forty differentially expressed proteins were found by image analysis software, and 24 differential proteins were identified by mass spectrometry. These proteins included proliferation cell nuclear antigen (PCNA), nm23, prohibitin (PHB), annexin, alpha-enolase, heat shock protein (HSP), and so on.
CONCLUSION
Twenty-four proteins in human ovarian cancer cell lines of paclitaxel-resistant and paclitaxel-sensitive are found by proteomic techniques, which may be involved in the paclitaxel-resistance of human ovarian cancer cells.
目的
研究卵巢癌细胞系中与紫杉醇耐药相关的蛋白质。
方法
采用二维凝胶电泳（2-DE）分离紫杉醇耐药和紫杉醇敏感的人卵巢癌细胞系的总蛋白。使用图像分析软件分析差异表达蛋白。使用基质辅助激光解吸/电离飞行时间质谱鉴定差异蛋白。采用蛋白质免疫印迹法测定这两种蛋白的差异表达水平。
结果
图像分析软件发现40种差异表达蛋白，质谱鉴定出24种差异蛋白。这些蛋白包括增殖细胞核抗原（PCNA）、nm23、抑制素（PHB）、膜联蛋白、α-烯醇化酶、热休克蛋白（HSP）等。
结论
通过蛋白质组学技术发现了紫杉醇耐药和紫杉醇敏感的人卵巢癌细胞系中的24种蛋白质，它们可能与人卵巢癌细胞的紫杉醇耐药有关。
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