Bioengineering Graduate Program, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
Chem Commun (Camb). 2010 Feb 28;46(8):1314-6. doi: 10.1039/b920696e. Epub 2010 Jan 15.
We report here a method to manipulate the amount of thiolated DNA attached per gold nanoparticle from a few to tens of strands in less than an hour, which involves the tuning of the respective amount of salt and time of addition of thiolated oligo(ethylene glycol) during mononucleotide-mediated conjugation process.
我们在此报告了一种方法,可在不到一个小时的时间内将每个金纳米粒子上连接的硫醇化 DNA 的数量从几个到几十个进行操纵,该方法涉及在单核苷酸介导的偶联过程中调整盐的各自用量和硫醇化聚(乙二醇)的添加时间。