Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan, 666303, China.
Protoplasma. 2011 Apr;248(2):251-6. doi: 10.1007/s00709-010-0154-4. Epub 2010 May 7.
The subcellular localization of calcium in Alpinia mutica Roxb. during style movement was studied in two morphs. In the styles, Ca-antimonate precipitates (ppts) were principally located in apoplasts, with some minimal accumulation in the nucleus. At different movement, stages of movement, the ppts in the abaxial and adaxial sides changed, and no lateral gradient of ppts in the apoplast was established. The increase or decrease of ppts in the apoplast was not accompanied with equivalent changes in the cytoplasm. These results indicate that calcium could not affect the curvature by inhibiting cell elongation but may play a role in style movement by acting as a secondary messenger. EGTA-treatment affected style movement, providing further evidence supporting a role for calcium as a secondary messenger.
在两种形态中研究了益智在花柱运动过程中钙的亚细胞定位。在花柱中，钙-锑酸盐沉淀（ppts）主要位于质外体中，核内有少量积累。在不同的运动阶段，ppts 在腹侧和背侧发生变化，质外体中没有建立 ppts 的侧梯度。质外体中 ppts 的增加或减少并不伴随着细胞质中相应的变化。这些结果表明，钙不能通过抑制细胞伸长来影响曲率，而是可能通过作为第二信使在花柱运动中发挥作用。EGTA 处理影响花柱运动，为钙作为第二信使的作用提供了进一步的证据。
