Hirst M C, Roche A, Flint T J, MacKinnon R N, Bassett J H, Nakahori Y, Watson J E, Bell M V, Patterson M N, Boyd Y
Molecular Genetics Group, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Genomics. 1991 May;10(1):243-9. doi: 10.1016/0888-7543(91)90506-a.
We have used recombinant clones derived from microdissection of the fragile X region to characterize breakpoints around the fragile site at Xq27.3. So far, no microdissection markers derived from Xq28 material have been found, thus allowing a rapid screening for clones surrounding the fragile site by their presence in a somatic cell hybrid containing Xq27.2-Xqter. A total of 43 new DNA markers from Xq27 have been sublocalized within this chromosome band. Of these new DNA markers, 5 lie in an interval defined as containing the fragile X region. The saturation of Xq27 with DNA markers by microdissection demonstrates the power of this technique and provides the resources for generating a complete physical map of the region.
