谷氨酸脱氢酶基因序列在小鼠7号和14号染色体上的染色体定位。

Chromosomal mapping of glutamate dehydrogenase gene sequences to mouse chromosomes 7 and 14.

作者信息

Tzimagiorgis G, Adamson M C, Kozak C A, Moschonas N K

机构信息

Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Crete, Greece.

出版信息

Genomics. 1991 May;10(1):83-8. doi: 10.1016/0888-7543(91)90487-y.

DOI:10.1016/0888-7543(91)90487-y

PMID:2045113

Abstract

Glutamate dehydrogenase (GLUD) plays an important role in mammalian neuronal transmission. In human, GLUD is encoded by a small gene family. To determine whether defects in Glud genes are associated with known neurological mutations in the mouse and to contribute to the comparative mapping of homologous genes in man and mouse, the chromosomal location of genes reactive with a mouse brain GLUD cDNA were determined. Genomic Southern analysis of a well-characterized panel of Chinese hamster x mouse somatic cell hybrids identified two GLUD-reactive loci, one residing on mouse Chromosome 14 and the other on Chromosome 7. Progeny of an intersubspecies backcross were used to map one of these genes, Glud, proximal to Np-1 on Chromosome 14, but no restriction fragment polymorphisms could be identified for the second locus, Glud-2.

摘要

谷氨酸脱氢酶（GLUD）在哺乳动物神经元传递中发挥着重要作用。在人类中，GLUD由一个小基因家族编码。为了确定Glud基因的缺陷是否与小鼠中已知的神经学突变相关，并有助于人类和小鼠同源基因的比较定位，确定了与小鼠脑GLUD cDNA反应的基因的染色体位置。对一组特征明确的中国仓鼠×小鼠体细胞杂种进行基因组Southern分析，确定了两个GLUD反应位点，一个位于小鼠第14号染色体上，另一个位于第7号染色体上。利用亚种间回交的后代将其中一个基因Glud定位在第14号染色体上Np-1的近端，但第二个位点Glud-2未发现限制性片段多态性。