Department of Biological Sciences, University of Texas, El Paso, TX, USA.
Mar Environ Res. 2010 Aug;70(2):133-41. doi: 10.1016/j.marenvres.2010.04.003. Epub 2010 Apr 24.
The present study investigated arsenic's effects on mummichogs (Fundulus heteroclitus), while also examining what role that gender or exposure age might play. Adult male and female mummichogs were exposed to 172 ppb, 575 ppb, or 1720 ppb arsenic as sodium arsenite for 10 days immediately prior to spawning. No differences were noted in the number or viability of eggs between the groups, but there was a significant increase in deformities in 1720 ppb arsenic exposure group. Total RNA from adult livers or 6-week old juveniles was used to probe custom macroarrays for changes in gene expression. In females, 3% of the genes were commonly differentially expressed in the 172 and 575 ppb exposure groups compared to controls. In the males, between 1.1 and 3% of the differentially expressed genes were in common between the exposure groups. Several genes, including apolipoprotein and serum amyloid precursor were commonly expressed in either a dose-responsive manner or were dose-specific, but consistent across genders. These patterns of regulation were confirmed by QPCR. These findings will provide us with a better understanding of the effects of dose, gender, and exposure age on the response to arsenic.
本研究调查了砷对花鲈（Fundulus heteroclitus）的影响，同时还研究了性别或暴露年龄可能起的作用。成年雌雄花鲈在产卵前 10 天分别暴露于 172 ppb、575 ppb 或 1720 ppb 砷酸钠中。各组之间的卵数或存活率没有差异，但在 1720 ppb 砷暴露组中畸形率显著增加。来自成年肝脏或 6 周龄幼鱼的总 RNA 用于探测定制的微阵列以检测基因表达的变化。在雌性中，与对照组相比，172 和 575 ppb 暴露组中有 3%的基因共同差异表达。在雄性中，暴露组之间有 1.1%至 3%的差异表达基因是共同的。一些基因，包括载脂蛋白和血清淀粉样蛋白前体，以剂量反应方式或剂量特异性表达，但在性别之间一致。这些调节模式通过 QPCR 得到了证实。这些发现将使我们更好地了解剂量、性别和暴露年龄对砷反应的影响。
