Department of Physical Chemistry and Materials Sciences, University of Szeged, Szeged, Hungary.
Colloids Surf B Biointerfaces. 2010 Aug 1;79(1):276-83. doi: 10.1016/j.colsurfb.2010.04.010. Epub 2010 Apr 21.
Lysozyme/gold thin layers were prepared by layer-by-layer (LbL) self-assembly method. The build-up of the films was followed by UV-vis-absorbance spectra, quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) techniques. The structural property of films was examined by X-ray diffraction (XRD) measurements, while their morphology was studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). It was found that gold nanoparticles (NPs) had cubic crystalline structure, the primary particles form aggregates in the thin layer due to the presence of lysozyme molecules. The UV-vis measurements prove change in particle size while the colour of the film changes from wine-red to blue. The layer thickness of films was determined using the above methods and the loose, porous structure of the films explains the difference in the results. The vapour adsorption property of hybrid layers was also studied by QCM using different saturated vapours and ammonia gas. The lysozyme/Au films were most sensitive for ammonia gas among the tested gases/vapours due to the strongest interaction between the functional groups of the protein.
溶菌酶/金薄膜是通过层层自组装方法制备的。通过紫外-可见吸收光谱、石英晶体微天平（QCM）和表面等离子体共振（SPR）技术对薄膜的构建进行了跟踪。通过 X 射线衍射（XRD）测量检查了薄膜的结构特性，通过扫描电子显微镜（SEM）和原子力显微镜（AFM）研究了其形态。结果发现，金纳米粒子（NPs）具有立方晶结构，由于溶菌酶分子的存在，初级粒子在薄膜中形成聚集体。紫外可见测量证明了粒径的变化，同时薄膜的颜色从酒红色变为蓝色。使用上述方法确定了薄膜的层厚，薄膜的疏松多孔结构解释了结果的差异。通过 QCM 使用不同的饱和蒸气和氨气研究了混合层的蒸气吸附特性。由于蛋白质的官能团之间存在最强的相互作用，因此在测试的气体/蒸气中，溶菌酶/Au 薄膜对氨气最敏感。
