Laboratory of Experimental Microbiology and National Institute for Translational Medicine (INCT-TM), Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil.
Neurosci Lett. 2010 Jul 5;478(2):93-6. doi: 10.1016/j.neulet.2010.04.072. Epub 2010 May 6.
Bacterial meningitis is associated with intense inflammation and also linked to the production of reactive oxygen species. To this aim, animals underwent a magna cistern tap and received either sterile saline as a placebo or an equivalent volume of a Streptococcus pneumoniae suspension. The animals began antibiotic therapy 16h after induction. The animals were sacrificed at 24 or 48h post-infection and the hippocampus and cortex were harvested. The activity of the enzymes superoxide dismutase, catalase, and thiobarbituric acid reactive species, protein carbonyls, and free sulphydryl groups were altered, but reversed, in part, by the antibiotic treatment. Our results support the hypothesis that antibiotic treatment prevents, in part, the oxidative stress in the bacterial meningitis induced by Streptococcus pneumoniae.
细菌性脑膜炎与剧烈的炎症有关，也与活性氧物质的产生有关。为此，动物接受了 magna 脑池穿刺，并接受了无菌生理盐水作为安慰剂或等量的肺炎链球菌混悬液。动物在诱导后 16 小时开始接受抗生素治疗。感染后 24 或 48 小时处死动物，并采集海马体和皮质。超氧化物歧化酶、过氧化氢酶和硫代巴比妥酸反应性物质、蛋白质羰基和游离巯基的活性发生改变，但抗生素治疗部分逆转了这种改变。我们的结果支持这样一种假设，即抗生素治疗部分预防了由肺炎链球菌引起的细菌性脑膜炎中的氧化应激。
