De Santo N G, Anastasio P, Coppola S, Capasso G, Bellini L, Spagnuolo G, Alfieri R, Massimo L, De Mercato R, Lombardi A
Department of Pediatrics, Università Federico II, Napoli, Italy.
Int J Artif Organs. 1991 Mar;14(3):161-8.
Ten patients with chronic renal failure (GFR 29-97 ml/min), on free diets providing 1 g/kg B.W. of proteins, ingested an oral protein load (meat meal, 2 g/kg B.W.). GFR and RPF increased significantly over baseline with no change in filtration fraction. Within 30 min of the meal and for the next 3 h a statistically significant increase was observed in the plasma concentrations of the following amino acid groups: essential, non-essential, total, branched-chain, ketogenic, glycogenic, glycogenic and ketogenic, basic, acid, polar and non-polar. At 30 min the smallest increase was seen in acid and polar amino acids (6.7% and 7.6%, respectively). At 180 min the largest increase (78.8%) was seen for glycogenic and ketogenic amino acids and total plasma amino acids were 1.58 times baseline. After the meat meal plasma glucagon and insulin rose significantly, while growth hormone, plasma renin activity and aldosterone did not vary.
十名慢性肾衰竭患者（肾小球滤过率为29 - 97毫升/分钟），采用自由饮食，蛋白质摄入量为1克/千克体重，摄入了一次口服蛋白质负荷量（肉粉，2克/千克体重）。肾小球滤过率和肾血浆流量较基线显著增加，滤过分数无变化。进食后30分钟内及随后3小时，观察到以下氨基酸组的血浆浓度有统计学意义的显著增加：必需氨基酸、非必需氨基酸、总氨基酸、支链氨基酸、生酮氨基酸、生糖氨基酸、生糖兼生酮氨基酸、碱性氨基酸、酸性氨基酸、极性氨基酸和非极性氨基酸。30分钟时，酸性和极性氨基酸的增加最小（分别为6.7%和7.6%）。180分钟时，生糖兼生酮氨基酸的增加最大（78.8%），血浆总氨基酸为基线的1.58倍。进食肉粉后，血浆胰高血糖素和胰岛素显著升高，而生长激素、血浆肾素活性和醛固酮无变化。
