Lloyd-Williams P, Albericio F, Giralt E
Department of Organic Chemistry, University of Barcelona, Spain.
Int J Pept Protein Res. 1991 Jan;37(1):58-60.
The solid-phase synthesis, photolytic detachment from the solid support and purification in solution, of a fully-protected octapeptide containing a methionine residue (protected as the sulphoxide) is described. Protection of methionine in this manner avoids problems associated with the oxidation of this residue during the photolysis. The peptide has been purified by medium pressure liquid chromatography using solvent mixtures containing a high proportion of dimethylformamide in order to avoid precipitation of the peptide on the column.
本文描述了一种含有甲硫氨酸残基（以亚砜形式保护）的全保护八肽的固相合成、从固相载体上的光解脱附以及在溶液中的纯化过程。以这种方式保护甲硫氨酸可避免在光解过程中该残基氧化相关的问题。该肽已通过中压液相色谱法纯化，使用了含有高比例二甲基甲酰胺的溶剂混合物，以避免肽在柱上沉淀。
