Departamento de Medicina Veterinária, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alto Universitário, s/n(o), Alegre/ES 29500-000, Brazil.
Micron. 2010 Aug;41(6):585-91. doi: 10.1016/j.micron.2010.04.005. Epub 2010 Apr 20.
Among the decapods, the caridean Exhippolysmata oplophoroides has been described as a simultaneous protandric hermaphrodite, seeing that it presents a male initial stage followed by a hermaphrodite one in which it can function as male and as female. This work had the aims of characterizing the microscopical morphology of the male portion of the ovotestes gonads from E. oplophoroides, at the different development stages, identifying each cell from the germ lines during spermatogenesis, as well as describing the ultramorphology of spermatozoans in the terminal region of the vasa deferentia. Shrimps were collected in Ubatuba, north coast of São Paulo, and their male gonads and the ampoule were removed, fixed and processed according to histological routine and for scanning electron microscopy. The testicular portion is divided in lobes, inside which cells at the same stage of the spermatogenic cycle are observed, with prevalence of spermatogonia and spermatocytes at stages I, II and V of gonad development, whereas spermatids and spermatozoans are found at stages III and IV, respectively. Ultramorphology of the terminal portion of the vasa deferentia exhibits mature aflagellated spike-shaped spermatozoans, encased in secretion and between membrane foldings that will constitute the spermatophores. Despite presenting reproductive characteristics common to other decapods, E. oplophoroides shows spermatozoans as well as spermatophore with typical morphology, which is important for its identification and taxonomy. Further, this species showed polysaccharide secretions where the spermatozoa are immerse as far as the testicular portion, which could have a role in their transport and nutrition as well as spermatophore constitution and/or fixation; differently, other caridean species begin spermatophore formation during the passage of the gametes through the vasa deferentia.
在十足目动物中，已经描述了 caridean Exhippolysmata oplophoroides 是一种同时进行的雌雄同体，因为它具有雄性初始阶段，随后是雌雄同体阶段，在该阶段中它可以作为雄性和雌性发挥功能。本工作旨在描述 E. oplophoroides 的卵睾卵巢雄性部分在不同发育阶段的微观形态，在精子发生过程中识别生殖系中的每个细胞，并描述输精管末端的精子超形态。虾类在圣保罗马北部海岸的乌巴图巴（Ubatuba）被收集，然后将其雄性性腺和精荚取出，按照组织学常规和扫描电子显微镜进行固定和处理。睾丸部分分为叶，在这些叶中观察到处于相同精子发生周期阶段的细胞，在性腺发育的阶段 I、II 和 V 中，以精原细胞和精母细胞为主，而在阶段 III 和 IV 中则发现精细胞和精子。输精管末端的超形态显示成熟的无鞭毛刺状精子，被包裹在分泌物中，并被膜折叠包裹，这些折叠将构成精荚。尽管 E. oplophoroides 具有与其他十足目动物共同的生殖特征，但它的精子和精荚具有典型的形态，这对于其鉴定和分类学非常重要。此外，该物种还表现出多糖分泌物，精子在这些分泌物中被包裹，一直延伸到睾丸部分，这可能在精子的运输和营养以及精荚的构成和/或固定中起作用；而其他 caridean 物种则在配子通过输精管时开始形成精荚。
