用于睡莲基因表达研究的候选参考基因。

Candidate reference genes for gene expression studies in water lily.

机构信息

Nanjing Agricultural University, Jiangsu, People's Republic of China.

出版信息

Anal Biochem. 2010 Sep 1;404(1):100-2. doi: 10.1016/j.ab.2010.05.002. Epub 2010 May 7.

DOI:10.1016/j.ab.2010.05.002

PMID:20452325

Abstract

The selection of an appropriate reference gene(s) is a prerequisite for the proper interpretation of quantitative Real-Time polymerase chain reaction data. We report the evaluation of eight candidate reference genes across various tissues and treatments in the water lily by the two software packages geNorm and NormFinder. Across all samples, clathrin adaptor complexes medium subunit (AP47) and actin 11 (ACT11) emerged as the most suitable reference genes. Across different tissues, ACT11 and elongation factor 1-alpha (EF1alpha) exhibited a stable expression pattern. ACT11 and AP47 also stably expressed in roots subjected to various treatments, but in the leaves of the same plants the most stably expressed genes were ubiquitin-conjugating enzyme 16 (UBC16) and ACT11.

摘要

选择合适的参考基因是正确解释定量实时聚合酶链反应数据的前提。我们报告了通过 geNorm 和 NormFinder 这两个软件包评估在各种组织和处理中在睡莲中 8 个候选参考基因的情况。在所有样品中，网格蛋白衔接蛋白复合物中等亚基（AP47）和肌动蛋白 11（ACT11）是最适合的参考基因。在不同组织中，ACT11 和延伸因子 1-α（EF1alpha）表现出稳定的表达模式。ACT11 和 AP47 在受到各种处理的根中也稳定表达，但在同一植物的叶子中，最稳定表达的基因是泛素连接酶 16（UBC16）和 ACT11。