Key Laboratory of Animal Epidemic Etiology & Immunological Prevention of Ministry of Agriculture, Zhejiang University, Hangzhou 310029, PR China.
Dev Comp Immunol. 2010 Sep;34(9):986-90. doi: 10.1016/j.dci.2010.04.008. Epub 2010 May 14.
Interleukin 2 (IL-2) plays an important role in the growth and differentiation of lymphocytes. To identify the functional domains of duck IL-2 (duIL-2), 4 neutralizing monoclonal antibodies (mAbs) to duIL-2 were used to finely map the functional domains of the duIL-2 protein. The mimotopes of 4 anti-duIL-2 mAbs, including LVXGSMPS, KPHKHHXHHSHM, WXXXKAKP, and HVPNERYPLR, were identified by phage display and peptide-competitive ELISA. These mimotopes constitute an important functional domain, Y(32) approximately T(44) (domain I), in the duIL-2 molecule. The bioactivity of the domain I peptide on in vitro lymphocyte proliferation was inhibited by duIL-2Ralpha. A tertiary structure model of duIL-2 showed that domain I is positioned in the long A-B loop and the N-terminal of Helix B. These data provided experimental evidence for elucidating the interaction between duIL-2 and duIL-2Ralpha.
白细胞介素 2（IL-2）在淋巴细胞的生长和分化中发挥重要作用。为了鉴定鸭白细胞介素 2（duIL-2）的功能域，使用 4 种针对 duIL-2 的中和单克隆抗体（mAb）来精细绘制 duIL-2 蛋白的功能域图谱。通过噬菌体展示和肽竞争 ELISA 鉴定了 4 种抗 duIL-2 mAb 的模拟表位，包括 LVXGSMPS、KPHKHHXHHSHM、WXXXKAKP 和 HVPNERYPLR。这些模拟表位构成了 duIL-2 分子中一个重要的功能域，Y（32）~T（44）（域 I）。该域 I 肽对体外淋巴细胞增殖的生物活性被 duIL-2Ralpha 抑制。duIL-2 的三级结构模型显示，域 I 位于长 A-B 环和螺旋 B 的 N 端。这些数据为阐明 duIL-2 与 duIL-2Ralpha 之间的相互作用提供了实验证据。
