CNR, Institute of Sciences of Food Production (ISPA), Via Amendola 122/O, 70126 Bari, Italy.
J Chromatogr A. 2010 Jun 25;1217(26):4300-5. doi: 10.1016/j.chroma.2010.04.035. Epub 2010 Apr 20.
A method based on capillary liquid chromatography combined with electrospray ionization-tandem mass spectrometry (CapLC-ESI-MS-MS) for the detection and identification of casein deriving peptides in fined white wine is described. This is the first step towards the development of a liquid chromatography mass spectrometric method for the detection/identification of markers of potentially allergenic milk proteins used as wine fining agents. The method demonstrated to be capable of detecting some peptides arising from alpha and beta casein (with the relative aminoacidic sequences elucidated) in extracts of white wine fined with casein at 100 and 1000 microg/mL. This MS based approach appears to be a useful tool for screening purposes as well as a confirmatory tool for the unequivocal identification of caseins in ELISA positive samples.
一种基于毛细管液相色谱-电喷雾串联质谱法（CapLC-ESI-MS-MS）的检测和鉴定精细白葡萄酒中酪蛋白衍生肽的方法。这是朝着开发用于检测/鉴定潜在过敏原性牛奶蛋白作为葡萄酒澄清剂的标记物的液相色谱-质谱方法迈出的第一步。该方法证明能够检测到用 100 和 1000μg/ml 酪蛋白澄清的白葡萄酒提取物中一些来自α和β酪蛋白的肽（阐明了相对氨基酸序列）。这种基于 MS 的方法似乎是一种有用的筛选工具，也是 ELISA 阳性样品中酪蛋白明确鉴定的确认工具。
