Department of Chemistry, University of Ferrara, Via L. Borsari, 46, I-44100 Ferrara, Italy.
J Chromatogr A. 2010 Jun 25;1217(26):4355-64. doi: 10.1016/j.chroma.2010.04.048. Epub 2010 Apr 24.
In this study, a comparative investigation was performed of HPLC Ascentis (2.7 microm particles) columns based on fused-core particle technology and Acquity (1.7 microm particles) columns requiring UPLC instruments, in comparison with Chromolith RP-18e columns. The study was carried out on mother and vegetal tinctures of Passiflora incarnata L. on one single or two coupled columns. The fundamental attributions of the chromatographic profiles are evaluated using a chemometric procedure, based on the AutoCovariance Function (ACVF). Different chromatographic systems are compared in terms of their separation parameters, i.e., number of total chemical components (m(tot)), separation efficiency (sigma), peak capacity (n(c)), overlap degree of peaks and peak purity. The obtained results show the improvements achieved by HPLC columns with narrow size particles in terms of total analysis time and chromatographic efficiency: comparable performance are achieved by Ascentis (2.7 microm particle) column and Acquity (1.7 microm particle) column requiring UPLC instruments. The ACVF plot is proposed as a simplified tool describing the chromatographic fingerprint to be used for evaluating and comparing chemical composition of plant extracts by using the parameters D% - relative abundance of the deterministic component - and c(EACF) - similarity index computed on ACVF.
在这项研究中，对基于熔融核颗粒技术的 HPLC Ascentis（2.7 微米颗粒）柱和需要 UPLC 仪器的 Acquity（1.7 微米颗粒）柱与 Chromolith RP-18e 柱进行了比较研究，与 Passiflora incarnata L. 的母液和植物酊剂在单个或两个耦合柱上进行了研究。使用基于自协方差函数（ACVF）的化学计量学程序评估色谱图的基本属性。根据分离参数，即总化学成分数量 (m(tot))、分离效率 (sigma)、峰容量 (n(c))、峰重叠度和峰纯度，比较了不同的色谱系统。研究结果表明，在总分析时间和色谱效率方面，具有窄粒径的 HPLC 柱取得了改进：可以通过 Ascentis（2.7 微米颗粒）柱和需要 UPLC 仪器的 Acquity（1.7 微米颗粒）柱实现可比的性能。提出 ACVF 图作为一种简化工具，用于描述色谱指纹，以使用参数 D%（确定性成分的相对丰度）和 c(EACF)（ACVF 上计算的相似指数）来评估和比较植物提取物的化学成分。
