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基于硅胶的整体柱在 HPLC 中的应用——分析生物样品中活性化合物的应用概述。

Silica-Based Monolithic Columns as a Tool in HPLC-An Overview of Application in Analysis of Active Compounds in Biological Samples.

机构信息

Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.

Faculty of Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University Lublin, 20-031 Lublin, Poland.

出版信息

Molecules. 2020 Jul 9;25(14):3149. doi: 10.3390/molecules25143149.

Abstract

Monolithic fillings used in chromatography are of great interest among scientists since the first reports of their synthesis and use were published. In the 20 years since silica-based monolithic columns were introduced into the commercial market, numerous papers describing their chromatographical properties and utility in various branches of industry and scientific investigations were presented. This review is focused on possible applications of commercially available silica-based HPLC monolithic columns in the analysis of biological samples.

摘要

整体式填充在色谱中引起了科学家们的极大兴趣,因为它们的合成和使用的最初报道已经发表。自硅胶整体柱进入商业市场以来的 20 年中,已经有许多描述其色谱性能以及在工业和科学研究各个领域中的应用的论文发表。本文综述了市售硅胶高效液相色谱整体柱在生物样品分析中的可能应用。

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本文引用的文献

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Are we approaching a post-monolithic era?我们是否正在进入后整体论时代?
J Sep Sci. 2020 May;43(9-10):1628-1633. doi: 10.1002/jssc.201901331. Epub 2020 Feb 9.
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Monolithic columns: A historical overview.整体柱:历史概述。
Electrophoresis. 2017 Nov;38(22-23):2810-2820. doi: 10.1002/elps.201700181. Epub 2017 Jul 5.

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