评估诱导突变策略在阿维菌素高产菌中的应用潜力。
Assessing the potential of an induced-mutation strategy for avermectin overproducers.
机构信息
Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100190, People's Republic of China.
出版信息
Appl Environ Microbiol. 2010 Jul;76(13):4583-6. doi: 10.1128/AEM.01682-09. Epub 2010 May 7.
Abstract
Mutant libraries of avermectin-producing Streptomyces avermitilis strains were constructed by different mutagenesis strategies. A metric was applied to assess the mutation spectrum by calculating the distribution of average phenotypic distance of each population. The results showed for the first time that a microgravity environment could introduce larger phenotype distribution and diversity than UV and N-methyl-N-nitro-N-nitrosoguanidine (NTG) could.
摘要
通过不同的诱变策略构建了阿维链霉菌产阿维菌素突变体库。通过计算每个群体平均表型距离的分布，应用一种度量标准来评估突变谱。结果首次表明，微重力环境可以比 UV 和 N-甲基-N-硝基-N-亚硝基胍（NTG）引入更大的表型分布和多样性。
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