Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, People's Republic of China.
Appl Microbiol Biotechnol. 2010 Jan;85(4):1219-25. doi: 10.1007/s00253-009-2345-5. Epub 2009 Dec 2.
Avermectins produced by Streptomyces avermitilis are potent against a broad spectrum of nematode and arthropod parasites with low-level side effects on the host organisms. This study was designed to investigate a high-throughput screening strategy for the efficient identification of avermectin high-yield strains. The production protocol was miniaturized in 96 deep-well microplates. UV absorbance at 245 nm was used to monitor avermectin production. A good correlation between fermentation results in both 96 deep-well microplates and conventional Erlenmeyer flasks was observed. With this protocol, the production of avermectins was determined in less than 10 min for a full plate without compromising accuracy. The high-yield strain selected through this protocol was also tested in 360 m(3) batch fermentation with 1.6-fold improved outcome. Thus, the development of this protocol is expected to accelerate the selection of superior avermectin-producing strains.
阿维菌素由阿维链霉菌产生，对广谱的线虫和节肢动物寄生虫具有强大的作用，对宿主生物的副作用较低。本研究旨在探索一种高通量筛选策略，以有效地鉴定阿维菌素高产菌株。生产方案在 96 孔深孔板中进行了小型化。在 245nm 处的紫外吸光度用于监测阿维菌素的产生。在 96 孔深孔板和常规摇瓶中的发酵结果之间观察到了很好的相关性。使用此方案，在不影响准确性的情况下，整个平板的阿维菌素生产可在不到 10 分钟内完成。通过该方案筛选出的高产菌株也在 360 立方米批量发酵中进行了测试，结果提高了 1.6 倍。因此，该方案的开发有望加速优良阿维菌素生产菌株的选择。
