用于有氧和无氧条件下质粒转移的原位检测的双报告系统。
Dual reporter system for in situ detection of plasmid transfer under aerobic and anaerobic conditions.
机构信息
Department of Biological Sciences, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, P.O. Box 443051, Moscow, ID 83844-3051, USA.
出版信息
Appl Environ Microbiol. 2010 Jul;76(13):4553-6. doi: 10.1128/AEM.00226-10. Epub 2010 May 7.
Abstract
We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs are available as plasmids and mini-mariner transposons.
摘要
我们设计了一种新的遗传工具，用于检测在厌氧和需氧条件下的质粒转移。该系统基于 T7 RNA 聚合酶基因和 T7 启动子驱动的氧非依赖性绿色荧光蛋白 evoglow，单独或与红色荧光蛋白 DsRed 结合使用。构建体可作为质粒和小型水手转座子获得。
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