唾液酸在C1型肉毒杆菌毒素通过大鼠肠上皮细胞的转运过程中的作用。
Involvement of sialic acid in transport of serotype C1 botulinum toxins through rat intestinal epithelial cells.
作者信息
Inui Ken, Ito Hiroaki, Miyata Keita, Matsuo Tomohito, Horiuchi Ryohta, Ikeda Toshihiko, Watanabe Toshihiro, Ohyama Tohru, Niwa Koichi
机构信息
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan.
出版信息
J Vet Med Sci. 2010 Sep;72(9):1251-5. doi: 10.1292/jvms.10-0090. Epub 2010 Apr 28.
Clostridium botulinum produces a large toxin complex (L-TC) composed of neurotoxin (BoNT) and non-toxic proteins. In animal botulism, BoNT or L-TC is absorbed via the intestinal epithelium. To establish the cellular mechanisms of botulinum toxin absorption, we used cultured rat intestinal epithelial cells to test the binding and transport of serotype C1 BoNT and L-TC through the cell layers. BoNT and L-TC bound to and passed through the cell layers, with L-TC exhibiting larger binding and transport. Binding and transport of these toxins were inhibited by N-acetyl neuraminic acid or neuraminidase treatment of the cells. These results suggest that binding of serotype C1 BoNT and L-TC to sialic acid on the cells promoted their transport through intestinal epithelial cell layers.
肉毒梭菌产生一种由神经毒素（BoNT）和无毒蛋白质组成的大型毒素复合物（L-TC）。在动物肉毒中毒中，BoNT或L-TC通过肠上皮吸收。为了确定肉毒毒素吸收的细胞机制，我们使用培养的大鼠肠上皮细胞来测试C1型BoNT和L-TC通过细胞层的结合和转运。BoNT和L-TC与细胞层结合并穿过细胞层，L-TC表现出更大的结合和转运。这些毒素的结合和转运通过对细胞进行N-乙酰神经氨酸或神经氨酸酶处理而受到抑制。这些结果表明，C1型BoNT和L-TC与细胞上唾液酸的结合促进了它们通过肠上皮细胞层的转运。
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