Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain.
Sex Dev. 2010;4(3):155-61. doi: 10.1159/000309780. Epub 2010 May 10.
Several species of the South American genus Akodon present fully fertile XY females besides XX ones. To analyze the possibility of a Sry mutation as the cause of sex reversal in A. azarae and A. boliviensis, we determined the sequence of the Sry gene in 2 males and 3 XY females from each of these species. The Sry gene sequence was also studied in A. dolores, a species that does not have XY females. In inter-specific comparisons, the percentage identities with respect to the region analyzed varied between 96.8% and 97.9%. An ORF of 543 nucleotides was identified, and the predicted Sry proteins comprised 180 amino acids, with an HMG domain of 83 amino acids. Our results indicate that female sex reversal in A. azarae and A. boliviensis cannot be explained by sequence differences in the Sry region analyzed here, which includes the complete ORF and, together with previous results concerning the inheritance of the XY condition, show that Sry mutation is not the basis of sex reversal.
南美洲的 Akodon 属有几个物种除了 XX 型个体外，还存在完全可育的 XY 型雌性个体。为了分析 Sry 基因突变是否是 A. azarae 和 A. boliviensis 性反转的原因，我们测定了这两个物种的 2 只雄性和 3 只 XY 型雌性个体的 Sry 基因序列。我们还研究了不具有 XY 型雌性个体的 A. dolores 物种的 Sry 基因序列。在种间比较中，与所分析区域的百分比同一性在 96.8%到 97.9%之间变化。鉴定出一个 543 个核苷酸的 ORF，预测的 Sry 蛋白由 180 个氨基酸组成，具有 83 个氨基酸的 HMG 结构域。我们的结果表明，A. azarae 和 A. boliviensis 中的雌性性反转不能用我们在这里分析的 Sry 区域的序列差异来解释，该区域包括完整的 ORF，并且与先前关于 XY 条件遗传的结果一起表明，Sry 基因突变不是性反转的基础。
