Department of Medicinal Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Pharmacology. 2010;85(5):286-94. doi: 10.1159/000297508. Epub 2010 May 4.
We investigated the synergetic effects of glucocorticoid and histamine H1 receptor antagonists on an atopic dermatitis model. Hairless mice were used in this study and an atopic dermatitis model was made by repeated application of 2,4,6-trinitrochlorobenzene. The effects of glucocorticoid, histamine H1 receptor antagonists, and the simultaneous use of these drugs were investigated by measuring scratching behavior, skin symptoms and nerve growth factor (NGF) in the skin. Topical application of prednisolone significantly inhibited scratching behavior, skin symptoms and NGF contents in the skin by repeated application. Olopatadine also showed a significant effect on scratching behavior and NGF contents in the skin, whereas chlorpheniramine showed no significant inhibitory effect on these indices. Furthermore, the combined use of prednisolone and olopatadine potentiated the inhibition of scratching behavior, skin symptoms, and NGF in the skin. From these findings, olopatadine potentiated the inhibitory effect of prednisolone on the symptoms of atopic dermatitis by inhibiting NGF.
我们研究了糖皮质激素和组胺 H1 受体拮抗剂对特应性皮炎模型的协同作用。本研究使用无毛小鼠，通过反复应用 2,4,6-三硝基氯苯制造特应性皮炎模型。通过测量搔抓行为、皮肤症状和皮肤中的神经生长因子（NGF）来研究糖皮质激素、组胺 H1 受体拮抗剂以及同时使用这些药物的效果。局部应用泼尼松龙可显著抑制重复应用引起的搔抓行为、皮肤症状和皮肤中 NGF 的含量。奥洛他定也对搔抓行为和皮肤中 NGF 的含量有显著的效果，而氯苯那敏对这些指标没有显著的抑制作用。此外，泼尼松龙和奥洛他定联合使用增强了对搔抓行为、皮肤症状和皮肤中 NGF 的抑制作用。从这些发现中可以看出，奥洛他定通过抑制 NGF 增强了泼尼松龙对特应性皮炎症状的抑制作用。
