Pérez-Merino Pablo, Martinez-Garcia M Carmen, Mar-Sardaña Santiago, Pérez-Escudero Alfonso, Blanco-Mezquita Tomas, Mayo-Iscar Agustin, Merayo-Lloves Jesus
Instituto Universitario de Oftalmobiología Aplicada, Department of Cell Biology, Faculty of Medicine, University of Valladolid, Valladolid, Spain.
Optom Vis Sci. 2010 Jul;87(7):E469-74. doi: 10.1097/OPX.0b013e3181e170df.
To determine the relation between the corneal light transmission measurements and the epithelial surface properties in hen corneas after different refractive surgery techniques photorefractive keratectomy, laser in situ keratomileusis, and laser-assisted subepithelial keratomileusis, and a group with only epithelial corneal removal (deepithelialization).
Five groups of hen corneas with different treatments and a control group were analyzed at 30 days. Direct transmittance and corneal light scattering were measured by a scatterometer developed by our group. Quantitative and systematic measurements of external and internal roughness and epithelium thickness were assessed using standard techniques developed for quantitative analysis of microphotographs of the corneal epithelium.
Data analysis revealed that the roughness in the epithelial surface was associated with the corneal light transmission. The direct transmittance of light showed a significant correlation with the epithelial roughness in the control (r = -0.99, p < 0.05) and photorefractive keratectomy (r = -0.99, p < 0.05) groups. However, there was no relation between the epithelial thickness and the corneal light transmission measurements.
The experimental results suggested that the roughness of the epithelial surfaces is related to the light transmission in the cornea.
确定在不同屈光手术技术（准分子激光角膜切削术、准分子原位角膜磨镶术和准分子激光上皮下角膜磨镶术）以及仅进行角膜上皮去除（去上皮化）的一组母鸡角膜中，角膜光传输测量值与上皮表面特性之间的关系。
在30天时对五组接受不同处理的母鸡角膜和一个对照组进行分析。使用我们小组开发的散射仪测量直接透过率和角膜光散射。使用为角膜上皮显微照片定量分析开发的标准技术，对外部和内部粗糙度以及上皮厚度进行定量和系统测量。
数据分析表明，上皮表面粗糙度与角膜光传输有关。在对照组（r = -0.99，p < 0.05）和准分子激光角膜切削术组（r = -0.99，p < 0.05）中，光的直接透过率与上皮粗糙度呈显著相关。然而，上皮厚度与角膜光传输测量值之间没有关系。
实验结果表明，上皮表面粗糙度与角膜中的光传输有关。
