Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brazil.
Can J Microbiol. 2010 Apr;56(4):342-7. doi: 10.1139/w10-011.
Bacteria from the genus Herbaspirillum are endophytes responsible for nitrogen fixation in gramineous plants of economic importance such as maize, sugarcane, sorghum, rice, and wheat. Some species are known to produce plant growth substances. In contrast, Herbaspirillum rubrisubalbicans strains are known to be mild plant pathogens. The molecular communication between the plant and the microbes might involve lipopolysaccharides present in the outer membrane of these gram-negative bacteria. Phenol-water extraction was used to obtain lipopolysaccharides from 7 strains of Herbaspirillum seropedicae (SmR1, Z67, Z78, ZA95, and M2) and H. rubrisubalbicans (M1 and M4). The electrophoretic profiles and chemical composition of the lipopolysaccharides obtained in the phenol and aqueous extracts were shown herein.
属于希瓦氏菌属的细菌是固氮菌，它们负责固定经济上重要的禾本科植物中的氮，如玉米、甘蔗、高粱、水稻和小麦。一些已知的物种能够产生植物生长物质。相比之下，希瓦氏菌rubrisubalbicans 菌株被认为是温和的植物病原体。植物和微生物之间的分子通讯可能涉及这些革兰氏阴性菌外膜中存在的脂多糖。本文采用酚-水提取法从 7 株希瓦氏菌属（SmR1、Z67、Z78、ZA95 和 M2）和希瓦氏菌 rubrisubalbicans （M1 和 M4）中提取脂多糖。本文展示了在酚和水提取物中获得的脂多糖的电泳图谱和化学组成。
