Tandem immunoaffinity and reversed-phase high-performance liquid chromatography for the identification of the specific binding sites of a hapten on a proteic carrier.
作者信息
Yvon M, Wal J M
机构信息
Station de Recherches Laitières, CRJ-INRA, Jouy-en-Josas, France.
出版信息
J Chromatogr. 1991 Feb 22;539(2):363-71. doi: 10.1016/s0021-9673(01)83945-5.
PMID:2045448
Abstract
Immunoaffinity (IA) and reversed-phase (RP) high-performance liquid chromatography were combined for the identification of the specific binding sites of benzylpenicilloyl (BPO) groups on human serum albumin (HSA). Tryptic hydrolysates of BPO-HSA were loaded on the IA column. BPO-peptides were desorbed and concentrated directly on the RP column, coupled via a switching valve, then separated by using gradient elution and identified by the amino acid sequences. This single-step procedure permitted more than 95% recovery of the BPO-peptides present in minute amounts, with good specificity.
摘要
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