Fletcher S, Neill W A, Norval M
Tawam Hospital Al Ain, Abu Dhabi, United Arab Emirates.
J Clin Pathol. 1991 May;44(5):410-5. doi: 10.1136/jcp.44.5.410.
The effects of several polyamines found in seminal fluid on the cell cycle and ploidy of three cervical cell lines and of primary epithelial cells cultured from cervical biopsy specimens were monitored by fluorescent flow cytometry. The rate of cell growth did not change but there were indications of either hypodiploidy or hyperdiploidy in some cultures at certain concentrations of spermine and spermidine. An interaction of exogenous polyamines with the DNA of cervical cells was shown to occur, leading to changes in ploidy with, perhaps, the potential to induce or promote dysplasia.
通过荧光流式细胞术监测精液中发现的几种多胺对三种宫颈细胞系以及从宫颈活检标本培养的原代表皮细胞的细胞周期和倍性的影响。细胞生长速率没有变化，但在某些浓度的精胺和亚精胺作用下，一些培养物中出现了亚二倍体或超二倍体的迹象。研究表明，外源性多胺与宫颈细胞的DNA发生相互作用，导致倍性变化，可能具有诱导或促进发育异常的潜力。
