Jain Richa, Jain P C
Department of Applied Microbiology and Biotechnology, Dr. Hari Singh Gour University, Sagar 470 003, India.
Indian J Exp Biol. 2010 Feb;48(2):174-8.
Streptomyces exfoliatus CFS 1068, an isolate of cultivated field soil, produced maximum collagenase activity (58.19 +/- 0.83 U ml-1 min-1) in 5 days when soybean meal and starch were used as nitrogen and carbon sources, respectively at pH 7 and 30 degrees C in shake cultures (150 rpm). Production of collagenase was higher (40.43 +/- 0.63 U ml(-1) min(-1)) when poultry feathers were used as nitrogen source. Thus, the strain was found to be of biotechnological importance. The purified enzyme showed 30.34 fold increase in collagenase activity and was stable at 70 degrees C for 1h. The enzyme was found to be of serine type.
从耕地土壤中分离得到的解淀粉链霉菌CFS 1068，在摇瓶培养（150转/分钟）中，以豆粕和淀粉分别作为氮源和碳源，pH值为7，温度为30℃的条件下，5天后产生的胶原酶活性最高（58.19±0.83 U ml-1 min-1）。当使用家禽羽毛作为氮源时，胶原酶的产量更高（40.43±0.63 U ml(-1) min(-1)）。因此，该菌株具有生物技术重要性。纯化后的酶的胶原酶活性提高了30.34倍，并且在70℃下稳定1小时。该酶被发现属于丝氨酸类型。
