Departamento de Bioloxía Vexetal e Ciencia do Solo, Facultade de Ciencias, Universidade de Vigo, Ourense, Spain.
FEMS Microbiol Ecol. 2010 Jul 1;73(1):149-56. doi: 10.1111/j.1574-6941.2010.00873.x. Epub 2010 Mar 30.
The effect of pH on the instantaneous growth of soil bacterial communities was studied in five soils with different pH (4.5-7.8) using leucine (Leu) and thymidine (TdR) incorporation. The pH dependency of bacterial growth was modelled using three different unimodal functions, and the pH(opt) for growth and the pH range in which growth was >50% of the optimal growth were compared. Leu and TdR incorporation yielded very similar results. The best fits were obtained using a third-degree polynomial function and the cardinal pH model. However, a simple second-degree function was adequate in most cases, yielding very similar pH(opt) values to the other two models. Bacterial growth was highly influenced by pH, showing optimum growth at a pH related to the soil pH. The lowest pH(opt) was found in the most acidic soil and the highest pH(opt) in the soil with the highest pH. The pH(opt) for bacterial growth was close to the soil pH measured in water, but higher (0.7-2.1 units) than the pH measured with 0.1 M KCl. The pH range in which bacterial growth was >50% of that at optimum was, on average, 1.7 units below and above the optimum pH.
采用亮氨酸（Leu）和胸苷（TdR）掺入法，研究了 5 种 pH 值（4.5-7.8）不同的土壤中 pH 对土壤细菌群落瞬时生长的影响。采用 3 种不同的单峰函数对细菌生长的 pH 值依赖性进行了建模，并比较了生长的最佳 pH 值（pH(opt)）和生长>最佳生长 50%的 pH 值范围。Leu 和 TdR 掺入得到了非常相似的结果。使用三次多项式函数和基数 pH 值模型可以得到最佳拟合。然而，在大多数情况下，简单的二次函数就足够了，与其他两种模型相比，得到了非常相似的 pH(opt)值。细菌生长受 pH 值的强烈影响，在与土壤 pH 值相关的 pH 值下表现出最佳生长。在最酸性的土壤中发现最低的 pH(opt)，在 pH 值最高的土壤中发现最高的 pH(opt)。细菌生长的最佳 pH 值接近用水测量的土壤 pH 值，但比用 0.1 M KCl 测量的 pH 值高 0.7-2.1 个单位。细菌生长>最佳生长 50%的 pH 值范围平均比最佳 pH 值低 1.7 个单位，比最佳 pH 值高 1.7 个单位。
Zotero 插件