Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, UK.
FEMS Microbiol Ecol. 2009 Dec;70(3):367-76. doi: 10.1111/j.1574-6941.2009.00748.x. Epub 2009 Jul 24.
Archaeal communities in many acidic forest soil systems are dominated by a distinct crenarchaeal lineage Group 1.1c. In addition, they are found consistently in other acidic soils including grassland pasture, moorland and alpine soils. To determine whether soil pH is a major factor in determining their presence and abundance, Group 1.1c community size and composition were investigated across a pH gradient from 4.5 to 7.5 that has been maintained for > 40 years. The abundances of Group 1.1c Crenarchaeota, total Crenarchaeota and total bacteria were assessed by quantitative PCR (qPCR) targeting 16S rRNA genes and the diversity of Group 1.1c crenarchaeal community was investigated by denaturing gradient gel electrophoresis (DGGE) and phylogenetic analysis. The abundance of Group 1.1c Crenarchaeota declined as the pH increased, whereas total Crenarchaeota and Bacteria showed no clear trend. Community diversity of Group 1.1c Crenarchaeota was also influenced with different DGGE bands dominating at different pH. Group 1.1c Crenarchaeota were also quantified in 13 other soils representing a range of habitats, soil types and pH. These results exhibited the same trend as that shown across the pH gradient with Group 1.1c Crenarchaeota representing a greater proportion of total Crenarchaeota in the most acidic soils.
在许多酸性森林土壤系统中，古菌群落主要由一个独特的古菌谱系 Group 1.1c 主导。此外，它们还普遍存在于其他酸性土壤中，包括草原牧场、荒野和高山土壤。为了确定土壤 pH 是否是决定其存在和丰度的主要因素，我们在 >40 年维持的 pH 梯度（从 4.5 到 7.5）上研究了 Group 1.1c 群落大小和组成。通过针对 16S rRNA 基因的定量 PCR(qPCR) 评估了 Group 1.1c 古菌、总古菌和总细菌的丰度，通过变性梯度凝胶电泳(DGGE)和系统发育分析研究了 Group 1.1c 古菌群落的多样性。随着 pH 的增加，Group 1.1c 古菌的丰度下降，而总古菌和细菌则没有明显的趋势。Group 1.1c 古菌群落的多样性也受到影响，不同的 DGGE 带在不同的 pH 下占主导地位。在代表一系列生境、土壤类型和 pH 的 13 种其他土壤中也定量了 Group 1.1c 古菌。这些结果与 pH 梯度上的结果相同，即 Group 1.1c 古菌在最酸性的土壤中代表了总古菌的更大比例。
