Department of Food Science, University of Udine, Udine, Italy.
FEMS Microbiol Lett. 2010 Jul 1;308(1):35-9. doi: 10.1111/j.1574-6968.2010.01984.x. Epub 2010 Apr 12.
Rainbow trout gastroenteritis has been related to the accumulation of segmented filamentous bacteria in the digestive tract of fish, which presents lethargy, reduced appetite and accumulation of mucoid faeces. Some authors associate the comparison of illness with the presence of viable filaments, which produce and release strings of endospores in the lumen of the gut. The segmented filamentous bacteria that could not be cultured in vitro have been related to Clostridium group I, and they have been named Candidatus arthromitus. Despite the various strategies that have been used to detect unculturable microorganisms, molecular methods have facilitated studies on culture-independent microorganisms. Direct DNA extraction from samples and subsequent study of 16S rRNA genes represent a tool for studying unculturable microbial flora. As direct detection of specific microorganisms is possible through the utilization of primers or probes annealing specific DNA sequences, the aim of this work was to design specific primers for the direct detection of C. arthromitus in fish using a nested PCR.
虹鳟鱼肠胃炎与鱼类消化道中分段丝状菌的积累有关，其表现为嗜睡、食欲减退和黏液性粪便堆积。一些作者将疾病的发生与活丝的存在联系起来，这些活丝在肠道腔中产生并释放出内孢子的链。无法在体外培养的分段丝状菌与 I 组梭菌有关，它们被命名为 Candidatus arthromitus。尽管已经使用了各种策略来检测不可培养的微生物，但分子方法促进了对非培养微生物的研究。直接从样品中提取 DNA，然后研究 16S rRNA 基因，是研究不可培养微生物菌群的一种工具。由于通过利用退火特定 DNA 序列的引物或探针可以直接检测特定的微生物，因此本工作旨在设计用于使用巢式 PCR 直接检测鱼类中 C. arthromitus 的特异性引物。
