Department of Microbiology, Dr R. M. L. Avadh University, Faizabad (UP), India.
J Appl Microbiol. 2010 Sep;109(3):1035-43. doi: 10.1111/j.1365-2672.2010.04731.x.
To isolate the novel nonmelanin pullulan-producing fungi from soil and to optimize the physico-chemical and nutritional parameters for pullulan production.
A selective enrichment method was followed for the isolation, along with development of a suitable medium for pullulan production, using shake flask experiments. Pullulan content was confirmed using pure pullulan and pullulanase hydrolysate. Eurotium chevalieri was able to produce maximum pullulan (38 ± 1·0 g l(-1) ) at 35°C, pH 5·5, 2·5% sucrose, 0·3% ammonium sulfate and 0·2% yeast extract in a shake flash culture medium with an agitation rate of 30 rev min(-1) for 65 h.
The novel pullulan-producing fungus was identified as E. chevalieri (MTCC no. 9614), which was able to produce nonmelanin pullulan at from poorer carbon and nitrogen sources than Aureobasidium pullulans and may therefore be useful for the commercial production of pullulan.
Eurotium chevalieri could produce pullulan in similar amounts to A. pullulans. Therefore, in future, this fungus could also be used for commercial pullulan production, because it is neither polymorphic nor melanin producing, hence its handling during pullulan fermentation will be easier and more economical.
从土壤中分离出新型非黑色素 pullulan 产生真菌，并优化 pullulan 生产的理化和营养参数。
采用选择性富集法进行分离，并通过摇瓶实验开发了一种适合 pullulan 生产的培养基。使用纯 pullulan 和 pullulanase 水解物确认 pullulan 含量。在摇瓶培养中，在 35°C、pH5.5、2.5%蔗糖、0.3%硫酸铵和 0.2%酵母提取物的条件下，以 30 rev min(-1)的搅拌速度培养 65 h，欧洲曲霉（MTCC 编号 9614）能够产生最大量的 pullulan（38±1.0 g l(-1)）。
新型 pullulan 产生真菌被鉴定为 E. chevalieri（MTCC 编号 9614），它能够从比 Aureobasidium pullulans 更差的碳源和氮源中产生非黑色素 pullulan，因此可能对 pullulan 的商业生产有用。
欧洲曲霉能够产生与 Aureobasidium pullulans 相当数量的 pullulan。因此，在未来，这种真菌也可以用于商业 pullulan 生产，因为它既非多态性也不产生黑色素，因此在 pullulan 发酵过程中处理起来会更容易且更经济。
