Tan Hua, Huang Xiao-ping, Deng Chang-qing
Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China.
Zhong Xi Yi Jie He Xue Bao. 2010 May;8(5):448-52. doi: 10.3736/jcim20100508.
To investigate the influence of astragaloside (AST) and Panax notoginseng saponins (PNS) combination on oxidative stress of brain tissues in C57BL/6 mice with cerebral ischemia-reperfusion injury.
Eighty C57BL/6 mice were randomly divided into sham-operated group, untreated group, high-dose combination group (AST at a dose of 220 mg/kg plus PNS at a dose of 230 mg/kg), medium-dose combination group (AST at a dose of 110 mg/kg plus PNS at a dose of 115 mg/kg), low-dose combination group (AST at a dose of 55 mg/kg plus PNS at a dose of 57.5 mg/kg), AST (110 mg/kg) group, PNS (115 mg/kg) group and edaravone (4 mg/kg) group. AST and PNS were administered by gavage once daily for 4 days and edaravone was administered by intraperitoneal injection twice daily for 4 days. On the fourth day, bilateral common carotid arteries were ligated for 20 minutes to induce cerebral ischemia, followed by 60 minutes of reperfusion. Ischemic brain tissue was used to prepare tissue homogenate, then contents of malonaldehyde (MDA), glutathione (GSH) and nitric oxide (NO), and activities of superoxide dismutase (SOD) and nitric oxide synthase (NOS) in the homogenate were detected. Two x two analysis of variance of factorial design was used to analyze whether there was an interaction between AST at 110 mg/kg and PNS at 115 mg/kg.
Compared with sham-operated group, contents of MDA and NO, and activity of NOS in the untreated group were remarkably increased (P<0.01), activity of SOD and content of GSH were decreased (P<0.01). Compared with the untreated group, content of MDA in the AST group was decreased (P<0.01) and activity of SOD was increased (P<0.01), however, contents of GSH and NO and activity of NOS had no obvious changes (P>0.05). Contents of MDA and NO in the PNS group was decreased as compared with the untreated group (P<0.01), but activities of SOD and NOS and content of GSH had no changes (P>0.05). Contents of MDA and NO and activity of NOS in brain tissues in the edaravone group were decreased (P<0.01, P<0.05), and activity of SOD was increased (P<0.05), while content of GSH had no changes (P>0.05). Contents of MDA and NO and activity of NOS in brain tissue in the AST and PNS combination groups were decreased (P<0.01, P<0.05), the activity of SOD increased (P<0.01, P<0.05), the content of GSH increased (P<0.01, P<0.05), and activity of SOD and content of GSH were increased (P<0.01, P<0.05). The results of analysis of variance of factorial design showed that there were interactions between AST (110 mg/kg) and PNS (115 mg/kg) (P<0.01).
Combination of AST (110 mg/kg) and PNS (115 mg/kg) has a restraint effect on the early oxidative stress injury in the brain after ischemia-reperfusion, and the combination has a synergistic or additive effect.
探讨黄芪甲苷（AST）与三七总皂苷（PNS）联合应用对脑缺血再灌注损伤C57BL/6小鼠脑组织氧化应激的影响。
将80只C57BL/6小鼠随机分为假手术组、未处理组、高剂量联合组（AST 220 mg/kg加PNS 230 mg/kg）、中剂量联合组（AST 110 mg/kg加PNS 115 mg/kg）、低剂量联合组（AST 55 mg/kg加PNS 57.5 mg/kg）、AST（110 mg/kg）组、PNS（115 mg/kg）组和依达拉奉（4 mg/kg）组。AST和PNS每日灌胃1次，连续4天，依达拉奉每日腹腔注射2次，连续4天。第4天，结扎双侧颈总动脉20分钟诱导脑缺血，随后再灌注60分钟。取缺血脑组织制备组织匀浆，检测匀浆中丙二醛（MDA）、谷胱甘肽（GSH）、一氧化氮（NO）含量及超氧化物歧化酶（SOD）、一氧化氮合酶（NOS）活性。采用析因设计的两因素方差分析分析110 mg/kg AST与115 mg/kg PNS之间是否存在交互作用。
与假手术组比较，未处理组MDA、NO含量及NOS活性显著升高（P<0.01），SOD活性及GSH含量降低（P<0.01）。与未处理组比较，AST组MDA含量降低（P<0.01），SOD活性升高（P<0.01），而GSH、NO含量及NOS活性无明显变化（P>0.05）。PNS组MDA、NO含量较未处理组降低（P<0.01），但SOD、NOS活性及GSH含量无变化（P>0.05）。依达拉奉组脑组织MDA、NO含量及NOS活性降低（P<0.01，P<0.05），SOD活性升高（P<0.05），GSH含量无变化（P>0.05）。AST与PNS联合组脑组织MDA、NO含量及NOS活性降低（P<0.01，P<0.05），SOD活性升高（P<0.01，P<0.05），GSH含量升高（P<0.01，P<0.05）。析因设计方差分析结果显示，110 mg/kg AST与115 mg/kg PNS之间存在交互作用（P<0.01）。
AST（110 mg/kg）与PNS（115 mg/kg）联合应用对缺血再灌注后早期脑氧化应激损伤具有抑制作用，且联合应用具有协同或相加效应。
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