Mao Qi-qi, Sun Xu, Qiu Dong-ni, Fu Xiao-dong, Liu Yi, Wang Wen-jian
Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Zhong Xi Yi Jie He Xue Bao. 2010 May;8(5):453-7. doi: 10.3736/jcim20100509.
To investigate the effects of Fufang Jiangzhi No. 3, a compound traditional Chinese herbal medicine, on cholesterol-bile acid metabolism in rabbits with hypercholesterolemia and to explore the mechanism.
Twenty-four male New Zealand white rabbits were randomly assigned into normal control group, untreated group and Fufang Jiangzhi No. 3 group, with 8 rabbits in each group. Rabbits in the untreated group and Fufang Jiangzhi No. 3 group were fed high cholesterol diet to induce hypercholesterolemia. After 4-week treatment, serum total cholesterol and bile acid contents were assessed. Activity of cholesterol 7alpha-hydroxylase (CYP7A1) in liver tissues was measured by enzyme-linked immunosorbent assay. The expressions of CYP7A1, bile salt export pump (BSEP) and small heterodimer partner (SHP) mRNAs in liver tissues were observed by real-time fluorescent quantitative polymerase chain reaction.
Compared with the normal control group, serum total cholesterol and bile acid contents in the untreated group were increased (P<0.01). Activity of CYP7A1 and expression of CYP7A1 mRNA were decreased and expressions of BSEP and SHP mRNAs were increased in liver tissues in the untreated group as compared with the normal control group (P<0.01). Serum total cholesterol level, and expressions of BSEP and SHP mRNAs in the Fufang Jiangzhi No. 3 group were lower than those in the untreated group (P<0.01). The CYP7A1 activity and expression of CYP7A1 mRNA in the Fufang Jiangzhi No. 3 group were increased as compared with the untreated group (P<0.01), however, there was no significant difference in bile acid between the Fufang Jiangzhi No. 3 group and the untreated group.
Fufang Jiangzhi No. 3 can up-regulate the expression of CYP7A1 mRNA, raise the activity of CYP7A1, and inhibit the expressions of BSEP and SHP mRNAs to regulate the metabolism of total cholesterol in rabbits.
研究复方降脂3号（一种复方中药）对高胆固醇血症家兔胆固醇-胆汁酸代谢的影响并探讨其机制。
将24只雄性新西兰白兔随机分为正常对照组、未治疗组和复方降脂3号组，每组8只。未治疗组和复方降脂3号组的兔子喂食高胆固醇饮食以诱导高胆固醇血症。治疗4周后，评估血清总胆固醇和胆汁酸含量。采用酶联免疫吸附测定法检测肝组织中胆固醇7α-羟化酶（CYP7A1）的活性。通过实时荧光定量聚合酶链反应观察肝组织中CYP7A1、胆盐输出泵（BSEP）和小异二聚体伴侣（SHP）mRNA的表达。
与正常对照组相比，未治疗组血清总胆固醇和胆汁酸含量升高（P<0.01）。与正常对照组相比，未治疗组肝组织中CYP7A1活性和CYP7A1 mRNA表达降低，BSEP和SHP mRNA表达升高（P<0.01）。复方降脂3号组血清总胆固醇水平以及BSEP和SHP mRNA表达低于未治疗组（P<0.01）。与未治疗组相比，复方降脂3号组CYP7A1活性和CYP7A1 mRNA表达升高（P<0.01），然而，复方降脂3号组与未治疗组之间胆汁酸无显著差异。
复方降脂3号可上调CYP7A1 mRNA表达，提高CYP7A1活性，并抑制BSEP和SHP mRNA表达，从而调节家兔总胆固醇代谢。
