Centro de Investigación en Reproducción Caprina, Universidad Autónoma Agraria Antonio Narro, Periférico Raúl López Sánchez y Carretera a Sante Fe, Torreón, Coahuila, Mexico.
Horm Behav. 2010 Aug;58(3):473-7. doi: 10.1016/j.yhbeh.2010.05.002. Epub 2010 May 10.
The study was conducted on two consecutive years to determine whether ovulatory activity can be induced in anovulatory goats by exposing them to sexually active bucks for 4, 8, 12 or 16 h per day during 15 consecutive days. In experiment 1, females remained continuously in the experimental pens where they were in contact with males. One group remained isolated from males (controls) and four other groups were exposed to sexually active males for 4, 8, 12 or 16 h per day. In experiment 2, females were taken away to "resting" pens free of male odours between the periods of contact with bucks. They were allocated to 5 groups as in experiment 1. Ovulations were determined by progesterone plasma levels and transrectal ultrasonography. Pregnancy was determined by abdominal ultrasonography. In both experiments, more than 90% of females exposed to the bucks had at least one ovulation during the whole experiment whereas only 11 or 0% (experiments 1 and 2, respectively) did so in the control group (P<0.001). Furthermore, the proportion of females ovulating did not differ among groups depending on duration of contact with bucks (P>0.05). In both experiments, pregnancy rates were not affected by the daily duration of contact with males (P>0.05). To conclude, 4h of daily contact with sexually active males is sufficient to stimulate ovulatory activity in anovulatory goats and this effect is not due to the presence of olfactory cues from the males remaining in the pens.
这项研究连续进行了两年,目的是确定通过让不排卵的母羊每天与活跃的公羊接触 4、8、12 或 16 小时,连续 15 天,是否可以诱导它们排卵。在实验 1 中,母羊一直留在实验围栏中,与公羊接触。一组母羊与公羊隔离(对照组),其余四组母羊每天与活跃的公羊接触 4、8、12 或 16 小时。在实验 2 中,母羊在与公羊接触的时间段之间被带到没有公羊气味的“休息”围栏中。它们被分配到与实验 1 相同的 5 个组中。通过孕激素血浆水平和直肠超声检查确定排卵。通过腹部超声检查确定妊娠。在这两个实验中,超过 90%的接触公羊的母羊在整个实验期间至少有一次排卵,而对照组中只有 11%或 0%(实验 1 和 2 分别)(P<0.001)。此外,与公羊接触的持续时间不同,排卵的母羊比例在各组之间没有差异(P>0.05)。在这两个实验中,与公羊每天接触的持续时间都不会影响妊娠率(P>0.05)。总之,每天与活跃的公羊接触 4 小时足以刺激不排卵的母羊排卵,这种效果不是由于留在围栏中的公羊的嗅觉线索引起的。