Institute of Medical Biology, Chinese Academy of Medicine Science, Peking Union Medical College, Kunming 650118, PR China.
Biochimie. 2010 Aug;92(8):1024-30. doi: 10.1016/j.biochi.2010.04.025. Epub 2010 May 10.
VP22, a tegument protein of herpes simplex virus type 1 (HSV-1), is present in many copies in one virion and undergoes different types of post-translational modification. VP22 is believed to have certain functions in viral infection apart from virus assembly. Here we show that VP22 physically interacted with infected cell polypeptide 0 (ICP0) and colocalized in the nucleus, indicating that VP22 could be functionally involved in the modulation of viral transcription through interaction with ICP0. In the HSV-1 infection system and chloramphenicol acetyltransferase (CAT) transcriptional system, VP22-ICP0 interaction was confirmed to play a role in modulating the transcription of some viral genes and could be a factor in viral transcription, which is probably required in the transcriptional control of latent infection.
单纯疱疹病毒 1 型（HSV-1）的衣壳蛋白 VP22 存在于一个病毒粒子中的许多拷贝中，并经历不同类型的翻译后修饰。VP22 除了参与病毒组装外，还被认为在病毒感染中具有某些功能。在这里，我们显示 VP22 与感染细胞多肽 0（ICP0）发生物理相互作用，并在核内共定位，表明 VP22 可以通过与 ICP0 的相互作用在病毒转录的调节中发挥功能。在 HSV-1 感染系统和氯霉素乙酰转移酶（CAT）转录系统中，VP22-ICP0 相互作用被证实可以调节某些病毒基因的转录，并且可能是病毒转录的一个因素，这可能是潜伏感染转录控制所必需的。
