Budd Steven J, Hartnett M Elizabeth
Department of Ophthalmology, School of Medicine, University of North Carolina, 130 Mason Farm Rd, Campus Box 7040, Chapel Hill, NC 27599-7040, USA.
Arch Ophthalmol. 2010 May;128(5):589-95. doi: 10.1001/archophthalmol.2010.65.
To determine expression of vascular endothelial growth factor (VEGF), pigment epithelium-derived factor, and their respective receptors in retinas using a model of retinopathy of prematurity.
Retinas isolated from a 50/10 oxygen (inspired oxygen cycled between 50% oxygen and 10% oxygen every 24 hours)-induced rat model of retinopathy of prematurity (50/10 OIR model), and from room air-raised rat pups (RA) at birth, age 14 days (persistent peripheral avascular retina in the 50/10 OIR model and complete retinal vascularization in RA) and age 18 days (intravitreous neovascularization in the 50/10 OIR model) were analyzed for messenger RNA of VEGF(164), neuropilin 1, neuropilin 2, VEGF receptor 1, VEGF receptor 2, pigment epithelium-derived factor, and pigment epithelium-derived factor receptor by real-time polymerase chain reaction.
In the 50/10 OIR model compared with RA, fold changes in expression of VEGF(164), neuropilin 1, and neuropilin 2 were significantly increased at ages 14 and 18 days. A trend for increased fold change was noted in expression of VEGF receptor 2 at age 14 days and a significant increase at age 18 days in the 50/10 OIR model compared with RA. Pigment epithelium-derived factor receptor was significantly increased at age 14 days in the 50/10 OIR model compared with RA.
Increased expression of VEGF(164) and angiogenic receptors were found in association with both avascular retina at day 14 and intravitreous neovascularization at day 18 in a relevant model of retinopathy of prematurity.
Increased VEGF and angiogenic receptors may have a role in the development of peripheral avascular retina and stage 3 retinopathy of prematurity.
利用早产儿视网膜病变模型确定视网膜中血管内皮生长因子（VEGF）、色素上皮衍生因子及其各自受体的表达情况。
从出生时、14日龄（50/10氧诱导的早产儿视网膜病变大鼠模型中持续性周边无血管视网膜，以及正常饲养大鼠幼崽的完全视网膜血管化）和18日龄（50/10氧诱导的早产儿视网膜病变大鼠模型中的玻璃体内新生血管形成）的50/10氧（每24小时吸入氧在50%氧和10%氧之间循环）诱导的早产儿视网膜病变大鼠模型（50/10 OIR模型）以及正常饲养的大鼠幼崽（RA）中分离视网膜，通过实时聚合酶链反应分析VEGF(164)、神经纤毛蛋白1、神经纤毛蛋白2、VEGF受体1、VEGF受体2、色素上皮衍生因子和色素上皮衍生因子受体的信使核糖核酸。
与RA相比，在50/10 OIR模型中，14日龄和18日龄时VEGF(164)、神经纤毛蛋白1和神经纤毛蛋白2的表达倍数变化显著增加。与RA相比，50/10 OIR模型中14日龄时VEGF受体2表达倍数变化有增加趋势，18日龄时显著增加。与RA相比，50/10 OIR模型中14日龄时色素上皮衍生因子受体显著增加。
在相关的早产儿视网膜病变模型中，发现VEGF(164)和血管生成受体表达增加与14日龄时的无血管视网膜以及18日龄时的玻璃体内新生血管形成有关。
VEGF和血管生成受体增加可能在周边无血管视网膜的形成和3期早产儿视网膜病变中起作用。
