去帽激活因子 HPat 是一种从果蝇细胞中与 GW182 共纯化的新型因子。
The decapping activator HPat a novel factor co-purifying with GW182 from Drosophila cells.
机构信息
University of Vienna, Department of Biochemistry and Cell Biology, Vienna, Austria.
出版信息
RNA Biol. 2010 May-Jun;7(3):381-5. doi: 10.4161/rna.7.3.12088. Epub 2010 May 14.
Abstract
miRNAs post-transcriptionally regulate gene expression in many eukaryotes and thereby affect a wide range of biological processes. GW182 is a key factor in translation repression and mRNA degradation by miRNAs. In this study we investigate the potential interaction of GW182 and translation or mRNA degradation factors in Drosophila S2 cells. We have identified the decapping activator HP at as a novel factor co-purifying with GW182. Furthermore, we show that the C-terminal domain of GW182, important for gene silencing, is sufficient to form a complex with HP at. Our findings implicate a potential interaction of the miRNA effector component GW182 with the decapping machinery.
摘要
miRNAs 在许多真核生物中转录后调控基因表达，从而影响广泛的生物学过程。GW182 是 miRNA 介导的翻译抑制和 mRNA 降解的关键因子。在这项研究中，我们研究了 GW182 和翻译或 mRNA 降解因子在果蝇 S2 细胞中的潜在相互作用。我们已经鉴定出去帽激活因子 HP1 作为与 GW182 共纯化的新型因子。此外，我们还表明 GW182 的 C 末端结构域对于基因沉默是重要的，它足以与 HP1 形成复合物。我们的发现表明 miRNA 效应成分 GW182 与去帽机制之间可能存在相互作用。
相似文献
1
The decapping activator HPat a novel factor co-purifying with GW182 from Drosophila cells.去帽激活因子 HPat 是一种从果蝇细胞中与 GW182 共纯化的新型因子。
RNA Biol. 2010 May-Jun;7(3):381-5. doi: 10.4161/rna.7.3.12088. Epub 2010 May 14.
2
HPat a decapping activator interacting with the miRNA effector complex.HPat 是一种与 miRNA 效应物复合物相互作用的脱帽激活因子。
PLoS One. 2013 Aug 19;8(8):e71860. doi: 10.1371/journal.pone.0071860. eCollection 2013.
3
HPat provides a link between deadenylation and decapping in metazoa.HPat 在后生动物中提供了连接去腺苷酸化和去帽化的作用。
J Cell Biol. 2010 Apr 19;189(2):289-302. doi: 10.1083/jcb.200910141.
4
mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes.微小RNA（miRNA）和GW182介导的信使核糖核酸（mRNA）降解需要CCR4:NOT去腺苷酸化酶和DCP1:DCP2脱帽复合体。
Genes Dev. 2006 Jul 15;20(14):1885-98. doi: 10.1101/gad.1424106. Epub 2006 Jun 30.
5
Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing.miRNA介导的基因沉默中脱帽增强子的靶向特异性要求。
Genes Dev. 2007 Oct 15;21(20):2558-70. doi: 10.1101/gad.443107. Epub 2007 Sep 27.
6
miRISC recruits decapping factors to miRNA targets to enhance their degradation.miRISC 将去帽因子募集到 miRNA 靶标上来增强它们的降解。
Nucleic Acids Res. 2013 Oct;41(18):8692-705. doi: 10.1093/nar/gkt619. Epub 2013 Jul 17.
7
Dcp1a and GW182 Induce Distinct Cellular Aggregates and Have Different Effects on microRNA Pathway.Dcp1a和GW182诱导不同的细胞聚集体，并对微小RNA通路产生不同影响。
DNA Cell Biol. 2017 Jul;36(7):565-570. doi: 10.1089/dna.2017.3633. Epub 2017 May 10.
8
MicroRNAs silence gene expression by repressing protein expression and/or by promoting mRNA decay.微小RNA通过抑制蛋白质表达和/或促进信使核糖核酸降解来使基因表达沉默。
Cold Spring Harb Symp Quant Biol. 2006;71:523-30. doi: 10.1101/sqb.2006.71.013.
9
Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila.dGW182的多个独立结构域在果蝇的miRNA介导的抑制中发挥作用。
RNA. 2009 May;15(5):794-803. doi: 10.1261/rna.1364909. Epub 2009 Mar 20.
10
The GW/WG repeats of Drosophila GW182 function as effector motifs for miRNA-mediated repression.果蝇 GW182 的 GW/WG 重复序列作为 miRNA 介导的抑制作用的效应基序。
Nucleic Acids Res. 2010 Oct;38(19):6673-83. doi: 10.1093/nar/gkq501. Epub 2010 Jun 8.
引用本文的文献
1
Analysis of RNA Interference Lines Identifies New Functions of Maternally-Expressed Genes Involved in Embryonic Patterning in Drosophila melanogaster.对RNA干扰品系的分析揭示了果蝇胚胎模式形成中母源表达基因的新功能。
G3 (Bethesda). 2015 Mar 31;5(6):1025-34. doi: 10.1534/g3.115.017517.
2
Roles of mRNA fate modulators Dhh1 and Pat1 in TNRC6-dependent gene silencing recapitulated in yeast.mRNA命运调节因子Dhh1和Pat1在酵母中重现的TNRC6依赖性基因沉默中的作用。
J Biol Chem. 2015 Mar 27;290(13):8331-47. doi: 10.1074/jbc.M114.615088. Epub 2015 Feb 5.
3
HPat a decapping activator interacting with the miRNA effector complex.HPat 是一种与 miRNA 效应物复合物相互作用的脱帽激活因子。
PLoS One. 2013 Aug 19;8(8):e71860. doi: 10.1371/journal.pone.0071860. eCollection 2013.
4
miRISC recruits decapping factors to miRNA targets to enhance their degradation.miRISC 将去帽因子募集到 miRNA 靶标上来增强它们的降解。
Nucleic Acids Res. 2013 Oct;41(18):8692-705. doi: 10.1093/nar/gkt619. Epub 2013 Jul 17.
5
A genome-wide RNAi screen reveals that mRNA decapping restricts bunyaviral replication by limiting the pools of Dcp2-accessible targets for cap-snatching.全基因组 RNAi 筛选揭示，mRNA 去帽限制了 Dcp2 可接近的靶标用于帽抢夺的池，从而限制了 bunyaviral 的复制。
Genes Dev. 2013 Jul 1;27(13):1511-25. doi: 10.1101/gad.215384.113.
6
The conserved P body component HPat/Pat1 negatively regulates synaptic terminal growth at the larval Drosophila neuromuscular junction.保守的 P 体成分 HPat/Pat1 负调控幼虫果蝇神经肌肉连接处的突触末梢生长。
J Cell Sci. 2012 Dec 15;125(Pt 24):6105-16. doi: 10.1242/jcs.113043. Epub 2012 Oct 24.
本文引用的文献
1
Structural basis of binding of P-body-associated proteins GW182 and ataxin-2 by the Mlle domain of poly(A)-binding protein.GW182 和 ataxin-2 与 poly(A)-结合蛋白的 Mlle 结构域结合的结构基础。
J Biol Chem. 2010 Apr 30;285(18):13599-606. doi: 10.1074/jbc.M109.089540. Epub 2010 Feb 24.
2
Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation.人类 GW182-PABC 相互作用在 microRNA 介导的去腺苷酸化中的结构见解。
Nat Struct Mol Biol. 2010 Feb;17(2):238-40. doi: 10.1038/nsmb.1768. Epub 2010 Jan 24.
3
CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells.CCR4 使与 RNA 诱导的沉默复合物相关的 mRNA 去腺苷酸化，发生在人细胞中。
Mol Cell Biol. 2010 Mar;30(6):1486-94. doi: 10.1128/MCB.01481-09. Epub 2010 Jan 11.
4
Poly(A)-binding protein modulates mRNA susceptibility to cap-dependent miRNA-mediated repression.多聚（A）结合蛋白调节 mRNA 对帽子依赖的 miRNA 介导的抑制的敏感性。
RNA. 2010 Jan;16(1):239-50. doi: 10.1261/rna.1795410. Epub 2009 Nov 24.
5
Ago-TNRC6 triggers microRNA-mediated decay by promoting two deadenylation steps.AGO-TNRC6通过促进两个去腺苷酸化步骤触发微小RNA介导的降解。
Nat Struct Mol Biol. 2009 Nov;16(11):1160-6. doi: 10.1038/nsmb.1709. Epub 2009 Oct 18.
6
The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release.GW182的沉默结构域与PABPC1相互作用，以促进微小RNA靶标的翻译抑制和降解，并且是靶标释放所必需的。
Mol Cell Biol. 2009 Dec;29(23):6220-31. doi: 10.1128/MCB.01081-09. Epub 2009 Sep 21.
7
Drosophila miR2 primarily targets the m7GpppN cap structure for translational repression.果蝇miR2主要靶向m7GpppN帽状结构以抑制翻译。
Mol Cell. 2009 Sep 24;35(6):881-8. doi: 10.1016/j.molcel.2009.09.009.
8
Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.哺乳动物微小RNA诱导沉默复合体招募CAF1和多聚腺苷酸结合蛋白以影响多聚腺苷酸结合蛋白依赖性去腺苷酸化。
Mol Cell. 2009 Sep 24;35(6):868-80. doi: 10.1016/j.molcel.2009.08.004. Epub 2009 Aug 27.
9
microRNA-mediated messenger RNA deadenylation contributes to translational repression in mammalian cells.miRNA 介导的信使 RNA 去腺苷酸化有助于哺乳动物细胞中的翻译抑制。
PLoS One. 2009 Aug 27;4(8):e6783. doi: 10.1371/journal.pone.0006783.
10
A C-terminal silencing domain in GW182 is essential for miRNA function.GW182中的C末端沉默结构域对miRNA功能至关重要。
RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr 21.
Zotero 插件