University of Vienna, Department of Biochemistry and Cell Biology, Vienna, Austria.
RNA Biol. 2010 May-Jun;7(3):381-5. doi: 10.4161/rna.7.3.12088. Epub 2010 May 14.
miRNAs post-transcriptionally regulate gene expression in many eukaryotes and thereby affect a wide range of biological processes. GW182 is a key factor in translation repression and mRNA degradation by miRNAs. In this study we investigate the potential interaction of GW182 and translation or mRNA degradation factors in Drosophila S2 cells. We have identified the decapping activator HP at as a novel factor co-purifying with GW182. Furthermore, we show that the C-terminal domain of GW182, important for gene silencing, is sufficient to form a complex with HP at. Our findings implicate a potential interaction of the miRNA effector component GW182 with the decapping machinery.
miRNAs 在许多真核生物中转录后调控基因表达，从而影响广泛的生物学过程。GW182 是 miRNA 介导的翻译抑制和 mRNA 降解的关键因子。在这项研究中，我们研究了 GW182 和翻译或 mRNA 降解因子在果蝇 S2 细胞中的潜在相互作用。我们已经鉴定出去帽激活因子 HP1 作为与 GW182 共纯化的新型因子。此外，我们还表明 GW182 的 C 末端结构域对于基因沉默是重要的，它足以与 HP1 形成复合物。我们的发现表明 miRNA 效应成分 GW182 与去帽机制之间可能存在相互作用。
