Sandberg G, Ernström U, Nordlind K, Fredholm B B
Int Arch Allergy Appl Immunol. 1978;56(5):449-56. doi: 10.1159/000232055.
The cyclic AMP level and the uptake of 3H-thymidine were studied in short-term suspension cultures of guinea pig lymphoid cells. Spleen cells from animals immunized 3 days previously with typhoid vaccine were shown to differ in three respects from the normal cells: (1) The level of cyclic AMP was higher. (2) The cyclic AMP increase following addition of isoproterenol or adenosine was abolished. (3) Isoproterenol (10(-5) to 10(-4) M) stimulated the uptake of 3H-thymidine in cells from immunized animals, while the uptake into normal cells was inhibited by these concentrations. A higher dose of isoproterenol (10(-3) M) inhibited 3H-thymidine uptake in both immunized and normal cells. The results reveal certain biochemical alterations in splenic lymphocytes after immunization. An altered 3H-thymidine uptake may be caused by the modified cyclic AMP metabolism in cells from immunized animals.
在豚鼠淋巴细胞的短期悬浮培养物中研究了环磷酸腺苷（cAMP）水平和3H-胸腺嘧啶核苷的摄取。结果显示，3天前用伤寒疫苗免疫的动物的脾细胞在三个方面与正常细胞不同：（1）环磷酸腺苷水平较高。（2）添加异丙肾上腺素或腺苷后环磷酸腺苷的增加被消除。（3）异丙肾上腺素（10^(-5)至10^(-4) M）刺激免疫动物细胞中3H-胸腺嘧啶核苷的摄取，而这些浓度会抑制正常细胞的摄取。更高剂量的异丙肾上腺素（10^(-3) M）抑制免疫细胞和正常细胞中3H-胸腺嘧啶核苷的摄取。结果揭示了免疫后脾淋巴细胞的某些生化改变。免疫动物细胞中环磷酸腺苷代谢的改变可能导致3H-胸腺嘧啶核苷摄取的改变。
