National Institute of Crop Science, Kannondai 2-1-18, Tsukuba, 305-8518, Japan.
Amino Acids. 2010 Nov;39(5):1435-49. doi: 10.1007/s00726-010-0608-1. Epub 2010 May 11.
Flooding is a major problem for soybean crop as it reduces the growth and grain yield. To investigate the function of the soybean cell wall in the response to flooding stress, cell wall proteins were analyzed. Cell wall proteins from roots and hypocotyls of soybeans, which were germinated for 2 days and subjected to 2 days of flooding, were purified, separated by two-dimensional polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. Sixteen out of 204 cell wall proteins showed responses to flooding stress. Of these, two lipoxygenases, four germin-like protein precursors, three stem 28/31 kDa glycoprotein precursors, and one superoxide dismutase [Cu-Zn] were downregulated. A copper amine oxidase was found to have shifted from the basic to acidic zone following flooding stress. Based on these results, it was confirmed by the lignin staining that the lignification was suppressed in the root of soybean under the flooding stress. These results suggest that the roots and hypocotyls of soybean caused the suppression of lignification through decrease of these proteins by downregulation of reactive oxygen species and jasmonate biosynthesis under flooding stress.
flooding 是大豆作物的一个主要问题，因为它会降低生长和谷物产量。为了研究大豆细胞壁在应对水淹胁迫中的功能，对细胞壁蛋白进行了分析。从发芽 2 天且经历 2 天水淹的大豆根和下胚轴中纯化、分离细胞壁蛋白，用考马斯亮蓝染色后进行二维聚丙烯酰胺凝胶电泳。在 204 种细胞壁蛋白中，有 16 种对水淹胁迫有反应。其中，两种脂氧合酶、四种萌发相关蛋白前体、三种茎 28/31 kDa 糖蛋白前体和一种超氧化物歧化酶[Cu-Zn]下调。铜胺氧化酶在水淹胁迫后从碱性区转移到酸性区。根据这些结果，通过木质素染色证实，在水淹胁迫下，大豆的根部木质化受到抑制。这些结果表明，在水淹胁迫下，大豆的根和下胚轴通过下调活性氧和茉莉酸生物合成来减少这些蛋白质，从而抑制木质化。
