Afanas'eva K S, Zazhitskaia M O, Sivolob A V
Tsitol Genet. 2009 Nov-Dec;43(6):3-7.
The results are presented on comparison of the kinetics of DNA exit during neutral and alkaline variants of single cell gel electrophoresis. It has been shown that pre-incubation of samples in alkaline buffer makes impossible DNA exit during the neutral electrophoresis, in contrast to the alkaline conditions where DNA exit is very efficient. The conclusion has been made that the alkaline conditions induce a disruption of DNA matrix interactions. The hypothetical nature of these interactions is discussed. The results obtained suggest that the mechanisms of DNA exit in the course of neutral and alkaline electrophoresis are essentially different. In the case of the neutral electrophoresis the comet tails are formed by the relaxed loop domains while during the alkaline electrophoresis they are formed by single stranded DNA fragments, which are pulled out by one and from the coil that is not bound to the matrix.
结果呈现了单细胞凝胶电泳中性和碱性变体过程中DNA逸出动力学的比较情况。结果表明，与碱性条件下DNA高效逸出不同，在碱性缓冲液中预孵育样品会使中性电泳过程中DNA无法逸出。得出的结论是，碱性条件会导致DNA与基质相互作用的破坏。讨论了这些相互作用的假设性质。所获得的结果表明，中性和碱性电泳过程中DNA逸出的机制本质上是不同的。在中性电泳情况下，彗星尾由松弛的环域形成，而在碱性电泳过程中，它们由单链DNA片段形成，这些片段从与基质不结合的线圈中被拉出。
