Tsymbaliuk V I, Vasyl'ieva I H, Oleksenko N P, Chopyk N H, Tsiubko O I, Halanta O S
Tsitol Genet. 2009 Nov-Dec;43(6):52-7.
We have examined the mitogenic and differentiation potential and remyelination properties of human embryonic nerve cells in culture. After 1 month of cultivation without differentiation agents CNP-positive cells (the mitotically-active precursors of oligodendrocytes) were expanded at 3,6 times. At the same time the amount of GalC-positive cells (mature oligodendrocytes) remained low. So, the remyelination properties of embryonic nerve cells can be explained by high concentration of oligodendrocytes precursors. Cell population after cultivation maintained the increased remyelination potential by increasing the number of CNP-positive cells that was confirmed by using experimental demyelination.
我们已经研究了培养的人胚胎神经细胞的促有丝分裂和分化潜能以及髓鞘再生特性。在没有分化因子的情况下培养1个月后，CNP阳性细胞（少突胶质细胞的有丝分裂活性前体）扩增了3.6倍。与此同时，GalC阳性细胞（成熟少突胶质细胞）的数量仍然很低。因此，胚胎神经细胞的髓鞘再生特性可以通过高浓度的少突胶质细胞前体来解释。培养后的细胞群体通过增加CNP阳性细胞的数量保持了增强的髓鞘再生潜能，这一点通过实验性脱髓鞘得到了证实。
