Hu Xiaoqing, Li Weihua, Tian Fang, Wang Yuzhu, Song Weimin, Li Runsheng, Ding Xuncheng, Jin Taiyi
Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.
Wei Sheng Yan Jiu. 2010 Mar;39(2):231-4.
This study aims to explore the effects of dibutyl phthalate (DBP) on the gonad development of F1 generation male zebrafish.
Fourty-eight pairs of adult zebrafish were randomly divided into 4 groups of 12 pairs each: blank control group, 0.01% acetone solvent control group, 1250 microg/L DBP high-dose group, and 625 microg/L DBP low-dose group. After 30 days of exposure, zebrafish in each group were mated and the F1 generations were raised up to 180 dph in clean water. Twenty F1 male zebrafish in each group underwent gonadal index (GSI) analysis and gonad histopathology examination.
GSI of F1 male zebrafish in high-dose group (5.23% +/- 1.5%) was significantly lower than that in blank control (6.09% +/- 2.5%) or solvent control (6.08% +/- 1.2%) (P < 0.05). Histological examination on testis showed that DBP inhibited male gonadal development of the F1 offspring and led to abnormal seminiferous tubules with clear characteristics of extensive and significant proliferation of Leydig cells accompanied by low sperm count.
Gonadal development can be inhibited to different degrees by prenatal exposure to DBP in F1 male zebrafish, including abnormal seminiferous tubules, reduced sperm count as well as Leydig cell hyperplasia. This anti-estrogen activity of DBP in zebrafish is similar to those DBP-induced testicular effects observed in male rats, marmoset and human beings.
本研究旨在探讨邻苯二甲酸二丁酯（DBP）对F1代雄性斑马鱼性腺发育的影响。
48对成年斑马鱼随机分为4组，每组12对：空白对照组、0.01%丙酮溶剂对照组、1250μg/L DBP高剂量组和625μg/L DBP低剂量组。暴露30天后，每组斑马鱼进行交配，并将F1代在清洁水中饲养至180日龄。每组20条F1雄性斑马鱼进行性腺指数（GSI）分析和性腺组织病理学检查。
高剂量组F1雄性斑马鱼的GSI（5.23%±1.5%）显著低于空白对照组（6.09%±2.5%）或溶剂对照组（6.08%±1.2%）（P<0.05）。睾丸组织学检查表明，DBP抑制了F1代子代的雄性性腺发育，导致生精小管异常，具有明显的Leydig细胞广泛且显著增殖的特征，同时精子数量减少。
F1雄性斑马鱼产前暴露于DBP可不同程度地抑制性腺发育，包括生精小管异常、精子数量减少以及Leydig细胞增生。DBP在斑马鱼中的这种抗雌激素活性与在雄性大鼠、狨猴和人类中观察到的DBP诱导的睾丸效应相似。
