寡糖辅助小分子的直接免疫传感。

Oligosaccharide-assisted direct immunosensing of small molecules.

机构信息

Kobe Pharmaceutical University, 4-19-1, Motoyama-Kitamachi, Higashinada-ku, Kobe 658-8558, Japan.

出版信息

Anal Chem. 2010 Jun 1;82(11):4333-6. doi: 10.1021/ac100865p.

PMID:20459059

Abstract

"Sandwich-type" noncompetitive (immunometric) assays allow for high-sensitivity high-throughput macromolecule sensing and determination but cannot be used on small molecules (haptens). Here, we isolated single-chain Fv fragments from a phage-display library, which bound to complexes of particular haptens (vitamin D and A derivatives) with immobilized beta-cyclodextrin or beta-maltosyl residues, and formed ternary complexes. These scFvs enabled novel "semisandwich-type" immunometric assays of haptens with nanomole-range sensitivities.

摘要

"三明治型"非竞争（免疫测定）分析允许对大分子进行高灵敏度高通量的检测和测定，但不能用于小分子（半抗原）。在这里，我们从噬菌体展示文库中分离出单链 Fv 片段，该片段与固定化β-环糊精或β-麦芽糖残基结合的特定半抗原（维生素 D 和 A 衍生物）的复合物结合，并形成三元复合物。这些 scFv 使新型半抗原的“半三明治型”免疫测定分析具有纳摩尔级的灵敏度。