在恶臭假单胞菌 GPo1 中利用两段式连续培养系统同时合成两种共聚物。
Simultaneous biosynthesis of two copolymers in Pseudomonas putida GPo1 using a two-stage continuous culture system.
机构信息
Empa, Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Biomaterials, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland.
出版信息
Biomacromolecules. 2010 Jun 14;11(6):1488-93. doi: 10.1021/bm100118t.
The bacterial production of medium-chain-length polyhydroxyalkanoate (PHA) in continuous two-stage cultivation was investigated. Two chemostats were linked in sequence and Pseudomonas putida GPo1 was supplied with different concentrations of octanoic acid in the first and 10-undecenoic acid in the second reactor. PHA isolated from the second bioreactor was a blend of two copolymers typically consisting of poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) (PHO) and poly(3-hydroxy-10-undecenoate-co-3-hydroxy-8-nonenoate-co-3-hydroxy-6-heptenoate) (PHUE) with structural (monomeric) purities of 85-95 mol %, indicating simultaneous PHA accumulation and degradation inside the cell. The maximum PHA content was found to be 53.8% of total cell dry weight, which is very high for mcl-PHA.
连续两段式培养中细菌生产中链长度聚羟基烷酸（PHA）的研究。两个恒化器串联，在第一个恒化器中用不同浓度的辛酸，在第二个恒化器中用 10-十一烯酸为碳源，培养恶臭假单胞菌 GPo1。从第二个生物反应器中分离出的 PHA 是两种共聚物的混合物，通常由聚（3-羟基辛酸酯-co-3-羟基己酸酯）（PHO）和聚（3-羟基-10-十一烯酸酯-co-3-羟基-8-壬烯酸酯-co-3-羟基-6-庚烯酸酯）（PHUE）组成，结构（单体）纯度为 85-95 mol%，表明细胞内同时存在 PHA 的积累和降解。发现最大 PHA 含量为总细胞干重的 53.8%，对于 mcl-PHA 来说非常高。
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