Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
BMC Genomics. 2010 May 12;11:298. doi: 10.1186/1471-2164-11-298.
Cancer genomes display characteristic patterns of chromosomal imbalances, often with diagnostic and prognostic relevance. Therefore assays for genome-wide copy number screening and simultaneous detection of copy number alterations in specific chromosomal regions are of increasing importance in the diagnostic work-up of tumors.
We tested the performance of Multiplex Amplicon Quantification, a newly developed low-cost, closed-tube and high-throughput PCR-based technique for detection of copy number alterations in regions with prognostic relevance for neuroblastoma. Comparison with array CGH and the established Multiplex Ligation-dependent Probe Amplification method on 52 neuroblastoma tumors showed that Multiplex Amplicon Quantification can reliably detect the important genomic aberrations.
Multiplex Amplicon Quantification is a low-cost and high-throughput PCR-based technique that can reliably detect copy number alterations in regions with prognostic relevance for neuroblastoma.
癌症基因组显示出特征性的染色体失衡模式，通常具有诊断和预后意义。因此，用于全基因组拷贝数筛选以及特定染色体区域拷贝数改变的同时检测的检测方法在肿瘤的诊断中变得越来越重要。
我们测试了 Multiplex Amplicon Quantification 的性能，Multiplex Amplicon Quantification 是一种新开发的低成本、封闭管和高通量基于 PCR 的技术，用于检测神经母细胞瘤中具有预后相关性的区域的拷贝数改变。与 array CGH 和已建立的 Multiplex Ligation-dependent Probe Amplification 方法在 52 个神经母细胞瘤肿瘤上的比较表明，Multiplex Amplicon Quantification 可以可靠地检测到重要的基因组异常。
Multiplex Amplicon Quantification 是一种低成本、高通量的基于 PCR 的技术，可可靠地检测神经母细胞瘤中具有预后相关性的区域的拷贝数改变。
