大肠杆菌β-异丙基苹果酸脱氢酶的DNA指导体外合成
DNA-directed in vitro synthesis of Escherichia coli beta-isopropylmalate dehydrogenase.
作者信息
Kung H, Weissbach H
出版信息
J Biol Chem. 1978 Apr 10;253(7):2078-80.
PMID:204639
Abstract
The in vitro synthesis of beta-isopropylmalate dehydrogenase (EC 1.1.1.85), an enzyme involved in leucine biosynthesis, has been obtained using as template DNA from the hybrid plasmid (pLC1) which contains the Escherichia coli leucine operon. Enzyme synthesis in vitro is stimulated about 2-fold by guanosine-5'-diphosphate-3'-diphosphate and inhibited about 60% by 2 X 10(-4) M L-leucine.
摘要
利用含有大肠杆菌亮氨酸操纵子的杂交质粒（pLC1）的DNA作为模板，已实现了β-异丙基苹果酸脱氢酶（EC 1.1.1.85）的体外合成，该酶参与亮氨酸的生物合成。体外酶合成受到鸟苷-5'-二磷酸-3'-二磷酸的约2倍刺激，并受到2×10⁻⁴ M L-亮氨酸的约60%抑制。
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